Buyang Huanwu Decoction Exerts Cardioprotective Effects through Targeting Angiogenesis via Caveolin-1VEGF Signaling Pathway in Mice with Acute Myocardial Infarction
Joint Authors
Zheng, Qun
Bao, Xiao-Yi
Zhu, Peng-Chong
Tong, Qiang
Zhu, Jia-Zhen
Zhuang, Zhuang
Wang, Yan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-16
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide.
The idea of therapeutic angiogenesis in ischemic myocardium is a promising strategy for MI patients.
Buyang Huanwu decoction (BHD), a famous Chinese herbal prescription, exerted antioxidant, antiapoptotic, and anti-inflammatory effects, which contribute to cardio-/cerebral protection.
Here, we aim to investigate the effects of BHD on angiogenesis through the caveolin-1 (Cav-1)/vascular endothelial growth factor (VEGF) pathway in MI model of mice.
Materials and Methods.
C57BL/6 mice were randomly divided into 3 groups by the table of random number: (1) sham-operated group (sham, n=15), (2) AMI group (AMI+sham, n=20), and (3) BHD-treated group (AMI+BHD, n=20).
2,3,5-Triphenyltetrazolium chloride solution stain was used to determine myocardial infarct size.
Myocardial histopathology was tested using Masson staining and hematoxylin-eosin staining.
CD31 immunofluorescence staining was used to analyze the angiogenesis in the infarction border zone.
Western blot analysis, immunofluorescence staining, and/or real-time quantitative reverse transcription polymerase chain reaction was applied to test the expression of Cav-1, VEGF, vascular endothelial growth factor receptor 2 (VEGFR2), and/or phosphorylated extracellular signal-regulated kinase (p-ERK).
All statistical analyses were performed using the SPSS 20.0 software and GraphPad Prism 6.05.
Values of P<0.05 were considered as statistically significant.
Results and Conclusion.
Compared with the AMI group, the BHD-treated group showed a significant improvement in the heart weight/body weight ratio, echocardiography images, cardiac function, infarct size, Mason staining of the collagen deposition area, and density of microvessel in the infarction border zone (P<0.05).
Compared with the AMI group, BHD promoted the expression of Cav-1, VEGF, VEGFR2, and p-ERK in the infarction border zone after AMI.
BHD could exert cardioprotective effects on the mouse model with AMI through targeting angiogenesis via Cav-1/VEGF signaling pathway.
American Psychological Association (APA)
Zhu, Jia-Zhen& Bao, Xiao-Yi& Zheng, Qun& Tong, Qiang& Zhu, Peng-Chong& Zhuang, Zhuang…[et al.]. 2019. Buyang Huanwu Decoction Exerts Cardioprotective Effects through Targeting Angiogenesis via Caveolin-1VEGF Signaling Pathway in Mice with Acute Myocardial Infarction. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1203551
Modern Language Association (MLA)
Zhu, Jia-Zhen…[et al.]. Buyang Huanwu Decoction Exerts Cardioprotective Effects through Targeting Angiogenesis via Caveolin-1VEGF Signaling Pathway in Mice with Acute Myocardial Infarction. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1203551
American Medical Association (AMA)
Zhu, Jia-Zhen& Bao, Xiao-Yi& Zheng, Qun& Tong, Qiang& Zhu, Peng-Chong& Zhuang, Zhuang…[et al.]. Buyang Huanwu Decoction Exerts Cardioprotective Effects through Targeting Angiogenesis via Caveolin-1VEGF Signaling Pathway in Mice with Acute Myocardial Infarction. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1203551
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203551