Critical Role of HAX-1 in Promoting Avian Influenza Virus Replication in Lung Epithelial Cells
Joint Authors
Xing, Zheng
Li, Xue
Qu, Bingqian
He, Ganlin
Cardona, Carol J.
Song, Yongchun
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The PB1-F2 protein of influenza A virus has been considered a virulence factor, but its function in inducing apoptosis may be of disadvantage to viral replication.
Host mechanisms to regulate PB1-F2-induced apoptosis remain unknown.
We generated a PB1-F2-deficient avian influenza virus (AIV) H9N2 and found that the mutant virus replicated less efficiently in human lung epithelial cells.
The PB1-F2-deficient virus produced less apoptotic cells, indicating that PB1-F2 of the H9N2 virus promotes apoptosis, occurring at the early stage of infection, in the lung epithelial cells.
To understand how host cells regulate PB1-F2-induced apoptosis, we explored to identify cellular proteins interacting with PB1-F2 and found that HCLS1-associated protein X-1 (HAX-1), located mainly in the mitochondria as an apoptotic inhibitor, interacted with PB1-F2.
Increased procaspase-9 activations, induced by PB1-F2, could be suppressed by HAX-1.
In HAX-1 knockdown A549 cells, the replication of AIV H9N2 was suppressed in parallel to the activation of caspase-3 activation, which increased at the early stage of infection.
We hypothesize that HAX-1 promotes AIV replication by interacting with PB1-F2, resulting in the suppression of apoptosis, prolonged cell survival, and enhancement of viral replication.
Our data suggest that HAX-1 may be a promoting factor for AIV H9N2 replication through desensitizing PB1-F2 from its apoptotic induction in human lung epithelial cells.
American Psychological Association (APA)
Li, Xue& Qu, Bingqian& He, Ganlin& Cardona, Carol J.& Song, Yongchun& Xing, Zheng. 2018. Critical Role of HAX-1 in Promoting Avian Influenza Virus Replication in Lung Epithelial Cells. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203571
Modern Language Association (MLA)
Li, Xue…[et al.]. Critical Role of HAX-1 in Promoting Avian Influenza Virus Replication in Lung Epithelial Cells. Mediators of Inflammation No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1203571
American Medical Association (AMA)
Li, Xue& Qu, Bingqian& He, Ganlin& Cardona, Carol J.& Song, Yongchun& Xing, Zheng. Critical Role of HAX-1 in Promoting Avian Influenza Virus Replication in Lung Epithelial Cells. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203571
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203571