ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt cCaspase-3 Pathway
Joint Authors
Li, Xin
Fang, Fang
Gao, Ying
Tang, Geng
Xu, Weiqiang
Wang, Yihan
Kong, Ruoxian
Tuyihong, Ayixianguli
Wang, Zhicheng
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
During radiotherapy, reactive oxygen species- (ROS-) induced apoptosis is one of the main mechanism of radiation.
Based on KillerRed which can induce ROS burst in different cell substructures, here we hypothesized that KillerRed targeting mitochondria (mtKR) could induce ROS to enhance apoptosis by radiation.
In this study, empty vector, mtKR, and mtmCherry plasmids were successfully constructed, and mitochondrial localization were detected in COS-7 and HeLa cells.
After HeLa cells were transfected and irradiated by visible light and X-rays, ROS levels, mitochondrial membrane potential (Δψm), ATPase activities, adenosine triphosphate (ATP) content, apoptosis, and the expressions of mRNA and protein were measured, respectively.
Data demonstrated that the ROS levels significantly increased after light exposure, and adding extra radiation, voltage-dependent anion channel 1 (VDAC1) protein increased in the mitochondria, while Na+-K+ and Ca2+-Mg2+ ATPase activities, ATP content, and Δψm significantly reduced.
Additionally, the cell apoptotic rates dramatically increased, which referred to the increase of cytochrome c (Cyt c), caspase-9, and caspase-3 mRNA expressions, and Cyt c protein was released from the mitochondria into the cytoplasm; caspase-9 and -3 were activated.
These results indicated that mtKR can increase the production of ROS, enhance mitochondrial dysfunction, and strengthen apoptosis by radiation via Cyt c/caspase-3 pathway.
American Psychological Association (APA)
Li, Xin& Fang, Fang& Gao, Ying& Tang, Geng& Xu, Weiqiang& Wang, Yihan…[et al.]. 2019. ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt cCaspase-3 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1203605
Modern Language Association (MLA)
Li, Xin…[et al.]. ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt cCaspase-3 Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1203605
American Medical Association (AMA)
Li, Xin& Fang, Fang& Gao, Ying& Tang, Geng& Xu, Weiqiang& Wang, Yihan…[et al.]. ROS Induced by KillerRed Targeting Mitochondria (mtKR) Enhances Apoptosis Caused by Radiation via Cyt cCaspase-3 Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1203605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203605