![](/images/graphics-bg.png)
MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI
Joint Authors
Liu, Hui-Bin
Yuan, Ye
Hu, Juan
Du, Zhimin
Guo, Jing
Sun, Chuan
Yan, Xiu-Qing
Yu, Jie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Acute myocardial infarction (AMI) is the leading cause of sudden death worldwide.
MicroRNA-155 (miR-155) has been reported to target antiapoptotic genes in various diseases models, but the functional role of miR-155 in response to MI injury needs further investigations.
This study investigated the role of miR-155 in myocardial ischemia injury.
TUNEL and flow cytometry were performed to measure cell apoptosis.
Western blot analysis was employed to detect protein expressions of Bcl-2, XIAP, Bax, and caspase-3.
qRT-PCR was used to quantify miRNA levels.
We showed that miR-155 was dynamically elevated in murine hearts subjected to MI and in neonatal rat ventricular cardiomyocyte (NRVM) injury induced by hydrogen peroxide (H2O2).
In response to H2O2, the silencing of miR-155 using AMO-155 (antisense inhibitor oligodeoxyribonucleotides) significantly increased cell viability and reduced cell apoptosis.
Moreover, AMO-155 reversed the H2O2-induced downregulation of Bcl-2 and XIAP and upregulation of Bax and cleaved-caspase-3.
Further study revealed that AMO-155 resulted in a decrease of H2O2-induced JC-1-labelled monomeric cell number.
In addition, AMO-155 markedly decreased infarct size, ameliorated impaired cardiac function, and significantly reduced apoptotic cell percentages in MI mice heart.
The RNA-binding protein Quaking (QKI) was predicted as a target gene of miR-155 through bioinformatic analysis, and AMO-155 attenuated the downregulation of QKI in H2O2-treated cardiomyocytes and MI mice heart.
Knockdown of QKI by siRNA abolished the antiapoptotic effects of AMO-155.
Taken together, miR-155 is upregulated in the MI heart and NRVMs in response to H2O2 stress, and downregulating of miR-155 protects cardiomyocytes against apoptosis.
Mechanistically, it is probably due to the repression of QKI signaling pathway.
American Psychological Association (APA)
Guo, Jing& Liu, Hui-Bin& Sun, Chuan& Yan, Xiu-Qing& Hu, Juan& Yu, Jie…[et al.]. 2019. MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1203687
Modern Language Association (MLA)
Guo, Jing…[et al.]. MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1203687
American Medical Association (AMA)
Guo, Jing& Liu, Hui-Bin& Sun, Chuan& Yan, Xiu-Qing& Hu, Juan& Yu, Jie…[et al.]. MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1203687
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203687