Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model
Joint Authors
Li, Xuqi
Wang, Kang
Wei, Guangbing
Liang, Ting
Wu, Yunhua
Sun, Xuejun
Yu, Junhui
Chen, Zilu
Xu, Zhengshui
Shen, Rui
Zheng, Lu
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Among all the common complications that occur after abdominal surgery, intestinal adhesion is perhaps the most unpleasant one.
However, current methods to treat and prevent intestinal adhesion are limited; thus, exploring new methods to prevent and treat intestinal adhesion is greatly needed.
In this study, we demonstrated that Danhong injection (DHI) may be used as a promising method to prevent and treat intra-abdominal adhesion in a rat model.
Materials and Methods.
Forty-eight rats were randomly divided into six groups.
Except for the sham-operated group, all rats underwent cecal abrasion to establish an adhesion model.
After the operation, the rats in the DHI-treated groups received different doses of DHI via the tail vein daily, while the other group was treated with the same volume of saline solution.
Seven days after the operation, all rats were sacrificed, and the degree of adhesion was evaluated by Nair’s scoring system.
The extent of inflammation in the adhesion tissue was detected by HE staining and the expression of tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β).
The collagen deposition was assessed by Sirius red staining and α-SMA, MMP9, t-PA, and PAI-1 levels.
Oxidative stress was indicated by the level of reactive oxygen species (ROS) in adhesion tissues and by immunohistochemical labeling of Nrf2.
Furthermore, rat primary peritoneal mesothelial cells (RPMCs) were treated with H2O2 and DHI, and NF-κB phosphorylation was detected to illustrate the effect of DHI on oxidative stress.
Results.
The intra-abdominal adhesion scores were significantly decreased in the groups treated with a high dose of DHI compared with the control groups, and the degree of inflammation, fibrosis, and oxidative stress was also significantly decreased.
DHI treatment significantly reduced the levels of TNF-α, TGF-β1, and PAI and increased the expression levels of MMP9, Nrf2, and t-PA in the adhesion tissues.
ROS levels and NF-κB phosphorylation were significantly reduced in DHI-treated RPMCs compared with the control RPMCs.
Conclusion.
DHI alleviates the formation of postoperative intra-abdominal adhesions by inhibiting inflammation, collagen deposition, and oxidative stress in a rat model and may serve as a promising drug to prevent intra-abdominal adhesions.
American Psychological Association (APA)
Wu, Yunhua& Wei, Guangbing& Yu, Junhui& Chen, Zilu& Xu, Zhengshui& Shen, Rui…[et al.]. 2019. Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1203711
Modern Language Association (MLA)
Wu, Yunhua…[et al.]. Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1203711
American Medical Association (AMA)
Wu, Yunhua& Wei, Guangbing& Yu, Junhui& Chen, Zilu& Xu, Zhengshui& Shen, Rui…[et al.]. Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1203711
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203711