Photochemical Treatment of Drosophila APCs Can Eliminate Associated Viruses and Maintain the APC Function for Generating Antigen-Specific CTLs Ex Vivo
Joint Authors
Ye, Jun
Yang, Chunxia
Cai, Zeling
Shi, Weixing
Yu, Hong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Drosophila cells transfected with MHC class I and a number of costimulation molecules including B7.1, ICAM, LFA-3, and CD70 are potent antigen-presenting cells (APCs) for the generation of antigen-specific cytotoxic T cells (CTLs) in vitro.
Using Drosophila APCs, CTLs specific for melanoma antigens have been generated in vitro and adoptively transferred to melanoma patients.
However, the recent discovery that Drosophila cells can carry insect viruses raises the potential risk of Drosophila APCs transmitting xenogenic viruses to patient CTLs.
In this study, we have investigated photoreactive methods to inactivate insect viruses in APC.
A clinical grade psoralen compound, 8-MOP (UVADEX) in combination with UVA treatment (5 joules/cm2) can be used to inactivate Drosophila cell viruses.
UVADEX treatment is sufficient to inactivate insect viruses but does not affect the expression of MHC class I molecules and costimulation molecules on Drosophila APCs.
In fact, UVADEX treatment prevents Drosophila APC growth while maintaining APC function.
Furthermore, UVADEX-treated Drosophila APCs maintain or have enhanced APC function as determined by enhanced T cell activation, proliferation, and CTL generation.
Thus, the use of UVADEX-treated Drosophila APCs may provide a valuable tool for immunotherapy to generate tumor antigen-specific CTLs.
American Psychological Association (APA)
Ye, Jun& Yang, Chunxia& Cai, Zeling& Shi, Weixing& Yu, Hong. 2018. Photochemical Treatment of Drosophila APCs Can Eliminate Associated Viruses and Maintain the APC Function for Generating Antigen-Specific CTLs Ex Vivo. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1203715
Modern Language Association (MLA)
Ye, Jun…[et al.]. Photochemical Treatment of Drosophila APCs Can Eliminate Associated Viruses and Maintain the APC Function for Generating Antigen-Specific CTLs Ex Vivo. Mediators of Inflammation No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1203715
American Medical Association (AMA)
Ye, Jun& Yang, Chunxia& Cai, Zeling& Shi, Weixing& Yu, Hong. Photochemical Treatment of Drosophila APCs Can Eliminate Associated Viruses and Maintain the APC Function for Generating Antigen-Specific CTLs Ex Vivo. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1203715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203715