The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages
Joint Authors
Ha, In-Hyuk
Lee, Sang Kook
Lee, Jinho
Shin, Joon-Shik
Kim, Won Kyung
Chung, Hwa-Jin
Koh, Wonil
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Shinbaro3, a formulation derived from the hydrolysed roots of Harpagophytum procumbens var.
sublobatum (Engl.) Stapf, has been clinically used in the pharamacopuncture treatment of arthritis in Korea.
In the present study, Shinbaro3 inhibited NO generation in LPS-induced RAW 264.7 cells in a dose-dependent manner.
Shinbaro3 also downregulated the mRNA and protein expression of inflammatory mediators in a dose-dependent manner.
Three mechanisms explaining the effects of Shinbaro3 in RAW 264.7 cells were identified as follows: (1) inhibition of the extracellular signal-regulated kinase 1 and 2 (ERK1/2), stress-activated protein kinase (SAPK)/c-Jun N-terminal protein kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) pathways; (2) suppression of IκB kinase-α/β (IKK-α/β) phosphorylation and nuclear factor-kappa B (NF-κB) subunits in the NF-κB pathway, which are involved in MyD88-dependent signalling; and (3) downregulation of IFN-β mRNA expression via inhibition of interferon regulatory factor 3 (IRF3) and Janus-activated kinase 1 (JAK1)/signal transducer and activator of transcription 1 (STAT1) phosphorylation, which is involved in TRIF-dependent signalling.
Shinbaro3 exerted anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophage cells through modulation of the TLR4/MyD88 pathways, suggesting that Shinbaro3 is a novel anti-inflammatory therapeutic candidate in the field of pharmacopuncture.
American Psychological Association (APA)
Chung, Hwa-Jin& Koh, Wonil& Kim, Won Kyung& Shin, Joon-Shik& Lee, Jinho& Lee, Sang Kook…[et al.]. 2018. The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1203767
Modern Language Association (MLA)
Chung, Hwa-Jin…[et al.]. The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages. Mediators of Inflammation No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1203767
American Medical Association (AMA)
Chung, Hwa-Jin& Koh, Wonil& Kim, Won Kyung& Shin, Joon-Shik& Lee, Jinho& Lee, Sang Kook…[et al.]. The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1203767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203767