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Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury
Joint Authors
Xiang, Meng
Chen, Sifeng
Ye, Zhibin
Wang, Shun
Tian, Xiaoyu
Li, Yijun
Xue, Rong
Guan, Haochen
Lu, Meng
Xu, Huijun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-26
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Aims.
Treatment for acute kidney injury (AKI) is challenging.
Induced pluripotent stem cells (iPSCs) have great therapeutic potential.
This study sought to determine whether iPSCs attenuate AKI and the role of reactive oxygen species (ROS).
Results.
We intravenously injected isogenic iPSCs into mice 2 h after renal ischemia-reperfusion injury (IRI).
The cells were selectively trafficked to ischemia/reperfusion-injured kidney where they decreased kidney ROS and inflammatory cytokines and improved kidney function and morphology.
Pretreating the cells with ROS inhibitors before administration decreased iPSC engraftment and abolished the protective effect of iPSCs.
In contrast, pretreating iPSCs with hydrogen peroxide increased iPSC engraftment and therapeutic effect.
Although the intravenously administered iPSCs trafficked to the IRI kidney, the cells did not differentiate into proximal or distal tubular epithelial cells.
In vitro, the capabilities of the iPSC-released substances to promote proliferation and decrease apoptosis of renal epithelial cells were increased by ROS pretreatment of iPSCs.
Moreover, pretreatment of the iPSCs with ROS inhibitor had the opposite effect.
Similarly, moderate concentrations of ROS increased while ROS inhibitors decreased iPSC mobility, adhesion to the extracellular matrix, and mitochondrial metabolism.
Innovation and Conclusion.
iPSCs decreased renal ischemia/reperfusion injury mainly through iPSC-released substances.
The therapeutic effect, mitochondrial metabolism, mobility, and kidney trafficking of iPSCs were ROS dependent.
American Psychological Association (APA)
Wang, Shun& Tian, Xiaoyu& Li, Yijun& Xue, Rong& Guan, Haochen& Lu, Meng…[et al.]. 2020. Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1203769
Modern Language Association (MLA)
Wang, Shun…[et al.]. Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1203769
American Medical Association (AMA)
Wang, Shun& Tian, Xiaoyu& Li, Yijun& Xue, Rong& Guan, Haochen& Lu, Meng…[et al.]. Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1203769
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203769