![](/images/graphics-bg.png)
High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats
Joint Authors
Novaes, Rômulo Dias
Gonçalves, Reggiani Vilela
Sarandy, Mariáurea Matias
Peluzio, Maria do Carmo Gouveia
Freitas, Mariella Bontempo
Rosa, Daiane Figueiredo
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The wound-healing process is complex and remains a challenging process under the influence of several factors, including eating habits.
As improper diets may lead to disorders such as dyslipidemia, insulin resistance, and chronic inflammation, potentially affecting the tissue ability to heal, we decided to investigate the effect of a high-fat diet and alcohol intake on the inflammatory process and skin wound healing in Wistar rats.
Male rats (n=30) were individually housed in cages with food and water ad libitum (registration number 213/2014).
After anesthesia, at day 40, three circular wounds (12 mm diameter) were made on the back of each animal, which were then randomly assorted into five treatment groups: C1 (control 1)—water via gavage and standard chow diet; C2 (control 2)—water (no gavage) and standard chow diet; AL (alcohol)—water (no gavage) and alcohol (40%) via gavage and standard chow diet; HF (high fat)—water (no gavage) and high-fat diet (50%); and HF + AL (alcohol/high fat)—water (no gavage), alcohol (40%) via gavage, and high-fat diet.
Animals were treated for 61 days.
Every seven days, the area and the rate of wound contraction were evaluated.
Tissue samples were removed for histopathological analysis and biochemical analyses.
Our results showed that wound contraction was not complete in the HF + AL rats.
Two specific indices of wound-healing impairment (total cell number and levels of the inflammatory cytokine TGF-β) were increased in the HF + AL rats.
We also observed decreased type I and III collagen fibers in the HF, AL, and HF + AL groups and increased oxidative stress markers in the same groups.
We suggest that a high-fat diet combined with alcohol intake contributed to delayed skin wound healing through increase of the inflammatory phase and promoting oxidative stress, which may have led to morphological alterations and impaired matrix remodeling.
American Psychological Association (APA)
Rosa, Daiane Figueiredo& Sarandy, Mariáurea Matias& Novaes, Rômulo Dias& Freitas, Mariella Bontempo& Peluzio, Maria do Carmo Gouveia& Gonçalves, Reggiani Vilela. 2018. High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203790
Modern Language Association (MLA)
Rosa, Daiane Figueiredo…[et al.]. High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats. Mediators of Inflammation No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1203790
American Medical Association (AMA)
Rosa, Daiane Figueiredo& Sarandy, Mariáurea Matias& Novaes, Rômulo Dias& Freitas, Mariella Bontempo& Peluzio, Maria do Carmo Gouveia& Gonçalves, Reggiani Vilela. High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203790
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203790