Tricetin Protects Rat Chondrocytes against IL-1β-Induced Inflammation and Apoptosis
Joint Authors
Sun, Fangfang
Hu, Peng-Fei
Xiong, Yan
Bao, Jia-Peng
Qian, Jing
Wu, Lidong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Tricetin is a well-studied flavonoid with a wide range of pharmacological activities in cancer and inflammation.
However, the ability of tricetin to ameliorate the inflammation that occurs in osteoarthritis (OA) has not been determined.
This study explored the effects of tricetin on interleukin- (IL-) 1β-induced rat chondrocytes.
Chondrocytes harvested from rat cartilage were incubated in vitro with tricetin in the presence of IL-1β.
The expression of matrix metalloproteinase- (MMP-) 1, MMP-3, MMP-13, nitric oxide (NO), prostaglandin E2 (PGE2), Bax, and Bcl-2 was evaluated by real-time-PCR, ELISA, Griess reaction, and western blotting.
Caspase-3 activity in chondrocytes was determined using a caspase-3 activity assay and MAPK pathway activity by western blotting.
Tricetin decreased the expression of MMP-1, MMP-3, and MMP-13 at both the gene and protein level in IL-1β-induced rat chondrocytes.
It also inhibited IL-1β-induced NO and PGE2 production, by modulating inducible NO synthase and cyclooxygenase 2 gene expression.
An antiapoptotic role of tricetin involving the Bax/Bcl-2/caspase-3 pathway was also determined.
The chondroprotective effect of tricetin was shown to be partly related to the suppression of the MAPK signaling pathway.
The results of this study demonstrate the chondroprotective role of tricetin, based on its anticatabolic, anti-inflammatory, and antiapoptotic effects in chondrocytes.
The therapeutic potential of tricetin in OA patients should be explored in future studies.
American Psychological Association (APA)
Sun, Fangfang& Hu, Peng-Fei& Xiong, Yan& Bao, Jia-Peng& Qian, Jing& Wu, Lidong. 2019. Tricetin Protects Rat Chondrocytes against IL-1β-Induced Inflammation and Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1203791
Modern Language Association (MLA)
Sun, Fangfang…[et al.]. Tricetin Protects Rat Chondrocytes against IL-1β-Induced Inflammation and Apoptosis. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1203791
American Medical Association (AMA)
Sun, Fangfang& Hu, Peng-Fei& Xiong, Yan& Bao, Jia-Peng& Qian, Jing& Wu, Lidong. Tricetin Protects Rat Chondrocytes against IL-1β-Induced Inflammation and Apoptosis. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1203791
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203791