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Anti-Inflammatory Effects of Aster incisus through the Inhibition of NF-κB, MAPK, and Akt Pathways in LPS-Stimulated RAW 264.7 Macrophages
Joint Authors
Ngabire, Daniel
Seong, Yeong-Ae
Patil, Maheshkumar Prakash
Niyonizigiye, Irvine
Seo, Yong Bae
Kim, Gun-Do
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aster incisus is a common flower found in almost all regions of South Korea.
In the current study, we investigated the potential antioxidant and anti-inflammatory properties of the Aster incisus methanol extract in LPS-stimulated RAW 264.7 cells.
We analyzed the phytochemicals contained in the extract by GC-MS.
GC-MS results showed that the Aster incisus extract contains 9 known compounds.
Later on, DPPH assay, WST-1 assay, nitric oxide (NO) assay, Western blot, and RT-PCR were conducted to investigate the anti-inflammatory effects of the extract.
Our WST-1 assay results revealed that Aster incisus did not affect the viability of all tested cell lines up to a concentration of 200 μg/ml; therefore, lower concentrations (50 μg/ml and 150 μg/ml) were used for further assays.
Aster incisus scavenged DPPH and inhibited the production of NO.
Aster incisus also reduced significantly the production of inflammation-related enzymes (iNOS, Cox-2) and cytokines (TNFα, IL-1β, and IL-6) and the gene expression of the proinflammatory cytokines.
Additionally, further Western blot results indicated that Aster incisus inhibited the expression of p-PI3K, p-IκBα, p-p65 NF-κB, p-ERK1/2, p-SAPK/JNK, and p-Akt.
Our results demonstrated that Aster incisus suppressed the expression of the inflammation mediators through the regulation of NF-κB, MAPK, and Akt pathways.
American Psychological Association (APA)
Ngabire, Daniel& Seong, Yeong-Ae& Patil, Maheshkumar Prakash& Niyonizigiye, Irvine& Seo, Yong Bae& Kim, Gun-Do. 2018. Anti-Inflammatory Effects of Aster incisus through the Inhibition of NF-κB, MAPK, and Akt Pathways in LPS-Stimulated RAW 264.7 Macrophages. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1203801
Modern Language Association (MLA)
Ngabire, Daniel…[et al.]. Anti-Inflammatory Effects of Aster incisus through the Inhibition of NF-κB, MAPK, and Akt Pathways in LPS-Stimulated RAW 264.7 Macrophages. Mediators of Inflammation No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1203801
American Medical Association (AMA)
Ngabire, Daniel& Seong, Yeong-Ae& Patil, Maheshkumar Prakash& Niyonizigiye, Irvine& Seo, Yong Bae& Kim, Gun-Do. Anti-Inflammatory Effects of Aster incisus through the Inhibition of NF-κB, MAPK, and Akt Pathways in LPS-Stimulated RAW 264.7 Macrophages. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1203801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203801