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Liproxstatin-1 Protects Hair Cell-Like HEI-OC1 Cells and Cochlear Hair Cells against Neomycin Ototoxicity
Joint Authors
Zheng, Zhiwei
Tang, Dongmei
Zhao, Liping
Li, Wen
Han, Jinghong
Hu, Bing
Nie, Guohui
He, Yingzi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-02
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Ferroptosis is a recently discovered iron-dependent form of oxidative programmed cell death distinct from caspase-dependent apoptosis.
In this study, we investigated the effect of ferroptosis in neomycin-induced hair cell loss by using selective ferroptosis inhibitor liproxstatin-1 (Lip-1).
Cell viability was identified by CCK8 assay.
The levels of reactive oxygen species (ROS) were determined by DCFH-DA and cellROX green staining.
The mitochondrial membrane potential (ΔΨm) was evaluated by TMRM staining.
Intracellular iron and lipid peroxides were detected with Mito-FerroGreen and Liperfluo probes.
We found that ferroptosis can be induced in both HEI-OC1 cells and neonatal mouse cochlear explants, as evidenced by Mito-FerroGreen and Liperfluo staining.
Further experiments showed that pretreatment with Lip-1 significantly alleviated neomycin-induced increased ROS generation and disruption in ΔΨm in the HEI-OC1 cells.
In parallel, Lip-1 significantly attenuated neomycin-induced hair cell damage in neonatal mouse cochlear explants.
Collectively, these results suggest a novel mechanism for neomycin-induced ototoxicity and suggest that ferroptosis inhibition may be a new clinical intervention to prevent hearing loss.
American Psychological Association (APA)
Zheng, Zhiwei& Tang, Dongmei& Zhao, Liping& Li, Wen& Han, Jinghong& Hu, Bing…[et al.]. 2020. Liproxstatin-1 Protects Hair Cell-Like HEI-OC1 Cells and Cochlear Hair Cells against Neomycin Ototoxicity. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1203820
Modern Language Association (MLA)
Zheng, Zhiwei…[et al.]. Liproxstatin-1 Protects Hair Cell-Like HEI-OC1 Cells and Cochlear Hair Cells against Neomycin Ototoxicity. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1203820
American Medical Association (AMA)
Zheng, Zhiwei& Tang, Dongmei& Zhao, Liping& Li, Wen& Han, Jinghong& Hu, Bing…[et al.]. Liproxstatin-1 Protects Hair Cell-Like HEI-OC1 Cells and Cochlear Hair Cells against Neomycin Ototoxicity. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1203820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203820