Preprotection of Tea Polysaccharides with Different Molecular Weights Can Reduce the Adhesion between Renal Epithelial Cells and Nano-Calcium Oxalate Crystals
Joint Authors
Ouyang, Jian-Ming
Sun, Xin-Yuan
Zhao, Yao-Wang
Liu, Li
Li, Chuang-Ye
Zhang, Hui
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Crystal adhesion is an important link in the formation of kidney stones.
This study investigated and compared the adhesion differences between nano-calcium oxalate monohydrate (COM) and human renal proximal tubule epithelial (HK-2) cells before and after treatment with tea polysaccharides (TPSs) TPS0, TPS1, TPS2, and TPS3 with molecular weights of 10.88, 8.16, 4.82, and 2.31 kDa, respectively.
TPS treatment effectively reduced the damage of COM to HK-2 cells, thereby resulting in increased cell activity, decreased release of lactate dehydrogenase, cell morphology recovery, decreased level of reactive oxygen species, increased mitochondrial membrane potential, increased lysosomal integrity, decreased expression of adhesion molecule osteopontin and eversion of phosphatidylserine, and decreased crystal adhesion.
Among the TPSs, TPS2 with moderate molecular weight had the best protective effect on cells and the strongest effect on the inhibition of crystal adhesion.
Thus, TPS2 may be a potential anticalculus drug.
American Psychological Association (APA)
Zhao, Yao-Wang& Liu, Li& Li, Chuang-Ye& Zhang, Hui& Sun, Xin-Yuan& Ouyang, Jian-Ming. 2020. Preprotection of Tea Polysaccharides with Different Molecular Weights Can Reduce the Adhesion between Renal Epithelial Cells and Nano-Calcium Oxalate Crystals. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1203831
Modern Language Association (MLA)
Zhao, Yao-Wang…[et al.]. Preprotection of Tea Polysaccharides with Different Molecular Weights Can Reduce the Adhesion between Renal Epithelial Cells and Nano-Calcium Oxalate Crystals. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1203831
American Medical Association (AMA)
Zhao, Yao-Wang& Liu, Li& Li, Chuang-Ye& Zhang, Hui& Sun, Xin-Yuan& Ouyang, Jian-Ming. Preprotection of Tea Polysaccharides with Different Molecular Weights Can Reduce the Adhesion between Renal Epithelial Cells and Nano-Calcium Oxalate Crystals. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1203831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203831