HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration
Joint Authors
Liu, Meidong
Tan, Chuyi
Liu, Yanjuan
Xiao, Gui
Tan, Sipin
Shi, Xueyan
Yin, Leijing
Deng, Huafei
Zhang, Huali
Xiao, Xianzhong
Gu, Jia
Li, Tao
Liu, Ke
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-18
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Heat shock factor 1 (HSF1) is a transcription factor involved in the heat shock response and other biological processes.
We have unveiled here an important role of HSF1 in acute lung injury (ALI).
HSF1 knockout mice were used as a model of lipopolysaccharide- (LPS-) induced ALI.
Lung damage was aggravated, and macrophage infiltration increased significantly in the bronchoalveolar lavage fluid (BALF) and lung tissue of HSF-/- mice compared with the damage observed in HSF1+/+ mice.
Upon LPS stimulation, HSF-/- mice showed higher levels of monocyte chemoattractant protein-1 (MCP-1) in the serum, BALF, and lung tissue and increased the expression of MCP-1 and chemokine (C-C motif) receptor 2 (CCR2) on the surface of macrophages compared with those in HSF1+/+.
Electrophoretic mobility shift assays (EMSA) and dual luciferase reporter assays revealed that HSF1 could directly bind to heat shock elements (HSE) in the promoter regions of MCP-1 and its receptor CCR2, thereby inhibiting the expression of both genes.
We concluded that HSF1 attenuated LPS-induced ALI in mice by directly suppressing the transcription of MCP-1/CCR2, which in turn reduced macrophage infiltration.
American Psychological Association (APA)
Li, Tao& Xiao, Gui& Tan, Sipin& Shi, Xueyan& Yin, Leijing& Tan, Chuyi…[et al.]. 2020. HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1203851
Modern Language Association (MLA)
Li, Tao…[et al.]. HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1203851
American Medical Association (AMA)
Li, Tao& Xiao, Gui& Tan, Sipin& Shi, Xueyan& Yin, Leijing& Tan, Chuyi…[et al.]. HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1203851
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203851