Repair of Tea Polysaccharide Promotes the Endocytosis of Nanocalcium Oxalate Monohydrate by Damaged HK-2 Cells
Joint Authors
Ouyang, Jian-Ming
Sun, Xin-Yuan
Zhao, Yao-Wang
Liu, Li
Li, Chuang-Ye
Peng, Qian-Long
Guo, Da
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Endocytosis is a protective mechanism of renal epithelial cells to eliminate retained crystals.
This research investigated the endocytosis of 100 nm calcium oxalate monohydrate crystals in human kidney proximal tubular epithelial (HK-2) cells before and after repair by four kinds of tea polysaccharides with molecular weights (MWs) of 10.88 (TPS0), 8.16 (TPS1), 4.82 (TPS2), and 2.31 kDa (TPS3), respectively.
When HK-2 cells were repaired by TPSs after oxalic acid injury, the cell viability, wound healing ability, mitochondrial membrane potential, percentage of cells with endocytosed crystals, and dissolution rate of the endocytosed crystals increased; the cell morphology recovered; and the reactive oxygen level and lactate dehydrogenase release decreased.
Most of the endocytosed crystals were found in the lysosomes.
The repair effects of the four TPSs were ranked in the following order: TPS2>TPS1>TPS3>TPS0.
TPS2 with moderate MW presented the optimal repair ability and strongest ability to promote endocytosis.
American Psychological Association (APA)
Li, Chuang-Ye& Liu, Li& Zhao, Yao-Wang& Peng, Qian-Long& Sun, Xin-Yuan& Guo, Da…[et al.]. 2020. Repair of Tea Polysaccharide Promotes the Endocytosis of Nanocalcium Oxalate Monohydrate by Damaged HK-2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1203898
Modern Language Association (MLA)
Li, Chuang-Ye…[et al.]. Repair of Tea Polysaccharide Promotes the Endocytosis of Nanocalcium Oxalate Monohydrate by Damaged HK-2 Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1203898
American Medical Association (AMA)
Li, Chuang-Ye& Liu, Li& Zhao, Yao-Wang& Peng, Qian-Long& Sun, Xin-Yuan& Guo, Da…[et al.]. Repair of Tea Polysaccharide Promotes the Endocytosis of Nanocalcium Oxalate Monohydrate by Damaged HK-2 Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1203898
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203898