Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress
Joint Authors
Tanimoto, Kotaro
Tanne, Yuki
Hirose, Naoto
Sumi, Chikako
Yanoshita, Makoto
Takano, Mami
Nishiyama, Sayuri
Okamoto, Yuki
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Excessive mechanical stress causes inflammation and destruction of cartilage and is considered one of the cause of osteoarthritis (OA).
Expression of semaphorin 3A (Sema3A), which is an axon guidance molecule, has been confirmed in chondrocytes.
However, there are few reports about Sema3A in chondrocytes, and the effects of Sema3A on inflammation in the cartilage are poorly understood.
The aim of this study was to examine the role of Sema3A in inflammation caused by high magnitude cyclic tensile strain (CTS).
Methods.
Expression of Sema3A and its receptors neuropilin-1 (NRP-1) and plexin-A1 (PLXA1) in ATDC5 cells was examined by Western blot analysis.
ATDC5 cells were subjected to CTS of 0.5 Hz, 10% elongation with added Sema3A for 3 h.
Gene expression of IL-1β, TNF-ɑ, COX-2, MMP-3, and MMP-13 was examined by qPCR analysis.
Furthermore, the phosphorylation of AKT, ERK, and NF-κB was detected by Western blot analysis.
Results.
Added Sema3A inhibited the gene expression of inflammatory cytokines upregulated by CTS in a dose-dependent manner.
Addition of Sema3A suppressed the activation of AKT, ERK, and NF-κB in a dose-dependent manner.
Conclusions.
Sema3A reduces the gene expression of inflammatory cytokines by downregulating the activation of AKT, ERK, and NF-κB pathways in ATDC5 cells under CTS.
American Psychological Association (APA)
Sumi, Chikako& Hirose, Naoto& Yanoshita, Makoto& Takano, Mami& Nishiyama, Sayuri& Okamoto, Yuki…[et al.]. 2018. Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1203902
Modern Language Association (MLA)
Sumi, Chikako…[et al.]. Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress. Mediators of Inflammation No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1203902
American Medical Association (AMA)
Sumi, Chikako& Hirose, Naoto& Yanoshita, Makoto& Takano, Mami& Nishiyama, Sayuri& Okamoto, Yuki…[et al.]. Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1203902
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203902