Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms
Joint Authors
Oliveira, Riva de Paula
de Oliveira Caland, Ricardo Basílio
Cadavid, Cesar Orlando Muñoz
Carmona, Lourdes
Peña, Leandro
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
‘Cara Cara’ is a red orange (Citrus sinensis (L.) Osbeck) variety originally from Venezuela characterized by a significantly higher and diversified carotenoid content including higher-concentration lycopene, all-E-β-carotene, phytoene, and other carotenoids when compared with the carotenoid profile of its isogenic blond counterpart ‘Bahia’, also known as Washington navel.
The exceptionally high carotenoid content of ‘Cara Cara’ is of special interest due to its neuroprotective potential.
Here, we used the nematode Caenorhabditis elegans to analyze the antioxidant effect and the protection against β-amyloid-induced toxicity of pasteurized orange juice (POJ) obtained from ‘Cara Cara’ and compare to that from ‘Bahia’.
POJ treatment reduced the endogenous ROS levels and increased the worm’s survival rate under normal and oxidative stress conditions.
POJ treatment also upregulated the expression of antioxidant (gcs-1, gst-4, and sod-3) and chaperonin (hsp-16.2) genes.
Remarkably, ROS reduction, gene expression activation, oxidative stress resistance, and longevity extension were significantly increased in the animals treated with ‘Cara Cara’ orange juice compared to animals treated with ‘Bahia’ orange juice.
Furthermore, the body paralysis induced by β-amyloid peptide was delayed by both POJs but the mean paralysis time for the worms treated with ‘Cara Cara’ orange juice was significantly higher compared to ‘Bahia’ orange juice.
Our mechanistic studies indicated that POJ-reduced ROS levels are primarily a result of the direct scavenging action of natural compounds available in the orange juice.
Moreover, POJ-induced gst-4::GFP expression and –increased stress resistance was dependent of the SKN-1/Nrf2 transcription factor.
Finally, the transcription factors SKN-1, DAF-16, and HSF-1 were required for the POJ-mediated protective effect against Aβ toxicity.
Collectively, these results suggest that orange juice from ‘Cara Cara’ induced a stronger response against oxidative stress and β-amyloid toxicity compared to orange juice from ‘Bahia’ possibly due to its higher carotenoid content.
American Psychological Association (APA)
de Oliveira Caland, Ricardo Basílio& Cadavid, Cesar Orlando Muñoz& Carmona, Lourdes& Peña, Leandro& Oliveira, Riva de Paula. 2019. Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1203908
Modern Language Association (MLA)
de Oliveira Caland, Ricardo Basílio…[et al.]. Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1203908
American Medical Association (AMA)
de Oliveira Caland, Ricardo Basílio& Cadavid, Cesar Orlando Muñoz& Carmona, Lourdes& Peña, Leandro& Oliveira, Riva de Paula. Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1203908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203908