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Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells
Joint Authors
Cavo, Michele
Sollazzo, Daria
Forte, Dorian
Romano, Marco
Vianelli, Nicola
Palandri, Francesca
Catani, Lucia
Barone, Martina
Allegri, Marisole
di Martella Orsi, Angela
Sinigaglia, Barbara
Auteri, Giuseppe
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-04
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Inflammation may play a role in cancer.
However, the contribution of cytokine-mediated crosstalk between normal hemopoietic stem/progenitor cells (HSPCs) and their (inflammatory) microenvironment is largely elusive.
Here we compared survival, phenotype, and function of neonatal (umbilical cord blood (CB)) and adult (normal G-CSF-mobilized peripheral blood (mPB)) CD34+ cells after in vitro exposure to combined crucial inflammatory factors such as interleukin- (IL-) 1β, IL-6, tumor necrosis factor- (TNF-) α, or tissue inhibitor of metalloproteinases-1 (TIMP-1).
To mimic bone marrow (BM) niche, coculture experiments with normal BM stromal cells (BMSCs) were also performed.
We found that combined inflammatory cytokines increased only the in vitro survival of CB-derived CD34+ cells by reducing apoptosis.
Conversely, selected combinations of inflammatory cytokines (IL-1β + TNF-α, IL-6 + TNF-α, and IL-1β + TNF-α + TIMP-1) mainly enhanced the in vitro CXCR4-driven migration of mPB-derived CD34+ cells.
TNF-α, alone or in combination, upregulated CD44 and CD13 expression in both sources.
Finally, BMSCs alone increased survival/migration of CB- and mPB-derived CD34+ cells at the same extent of the combined inflammatory cytokines; importantly, their copresence did not show additive/synergistic effect.
Taken together, these data indicate that combined proinflammatory stimuli promote distinct in vitro functional activation of neonatal or adult normal HSPCs.
American Psychological Association (APA)
Forte, Dorian& Sollazzo, Daria& Barone, Martina& Allegri, Marisole& di Martella Orsi, Angela& Romano, Marco…[et al.]. 2018. Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1203928
Modern Language Association (MLA)
Forte, Dorian…[et al.]. Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells. Mediators of Inflammation No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1203928
American Medical Association (AMA)
Forte, Dorian& Sollazzo, Daria& Barone, Martina& Allegri, Marisole& di Martella Orsi, Angela& Romano, Marco…[et al.]. Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1203928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203928