The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes
Joint Authors
Hseu, You Cheng
Yang, Hsin-Ling
Gowrisankar, Yugandhar Vudhya
Chen, Xuan-Zao
Yang, Yi-Chen
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
UVA irradiation induced ROS-mediated photo damage to the human skin leading to coarseness, wrinkling, pigmentation, and cutaneous malignancies.
We investigated the dermatoprotective efficacies of submicromolar concentrations of ergothioneine (EGT, 0.125-0.5 μM), which occurs naturally as a sulfur-containing amino acid, in the mechanisms in human skin fibroblast (HSF) cells.
UVA-induced AP-1 (c-Fos and c-Jun) translocation was found to be inhibited by EGT treatments with the parallel inhibition of the collagenolytic matrix metalloproteinase- (MMP-) 1 activation and type I procollagen degradation.
Moreover, EGT mitigated UVA-induced ROS generation.
An increase in the amount of antioxidant genes (HO-1, NQO-1, and γ-GCLC) from EGT and were associated with upregulated Nrf2 expressions in a dose-dependent or time-dependent manner.
We confirmed this from Nrf2 translocation and increased nuclear ARE promoter activity that underlie EGT dermatoprotective activities.
Also, glutathione (GSH) levels (from γ-GCLC) were significantly increased.
Moreover, we showed that mediated by ERK, JNK, and PKC, signaling cascades mediate Nrf2 translocation.
We confirmed this phenomenon by the suppressed nuclear Nrf2 activation in cells that were treated with respective inhibitors (PD98059, SP600125, and GF109203X).
However, antioxidant protein expressions were impaired in Nrf2 knockdown cells to confirm that ARE/Nrf2 pathways and the inhibition of AP-1 had significant roles in EGT-mediated protective effects.
We can conclude that ergothioneine ameliorated UVA-induced skin aging and is a useful food supplement for skin care products.
American Psychological Association (APA)
Hseu, You Cheng& Gowrisankar, Yugandhar Vudhya& Chen, Xuan-Zao& Yang, Yi-Chen& Yang, Hsin-Ling. 2020. The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204001
Modern Language Association (MLA)
Hseu, You Cheng…[et al.]. The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1204001
American Medical Association (AMA)
Hseu, You Cheng& Gowrisankar, Yugandhar Vudhya& Chen, Xuan-Zao& Yang, Yi-Chen& Yang, Hsin-Ling. The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204001