Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages
Joint Authors
Hsieh, Chia-Chien
Wang, Chih-Hsuan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The tumor microenvironment is rich in multiple cell types that influence tumor development.
Macrophages infiltrate tumors, where they are the most abundant immune cell population and secrete a number of cytokines.
Aspirin acts as a chemopreventive agent against cancer development.
This study investigated whether aspirin regulates crosstalk between breast cancer cells and macrophages.
To study these interactions in a tumor microenvironment, a conditioned media was employed using 4T1 breast cancer cells cultured in RAW 264.7 cell-conditioned medium (RAW-CM), and a cocultured model of both cells was used.
When 4T1 cells were cultured in the RAW-CM, there were increases in cell viability and secretion of the cytokines VEGF, PAI-1, TNF-α, and IL-6.
Treatment with aspirin inhibited 4T1 cell growth and migration and MCP-1, PAI-1, and IL-6 production.
In the coculture of both cells, aspirin inhibited secretion of MCP-1, IL-6, and TGF-β.
Furthermore, aspirin significantly decreased the M2 macrophage marker CD206, but increased M1 marker CD11c expression.
In summary, aspirin treatment inhibited the crosstalk of 4T1 and RAW 264.7 cells through regulation of angiogenic and inflammatory mediator production and influenced the M1/M2 macrophage subtype.
This highlighted that aspirin suppresses the tumor favorable microenvironment and could be a promising agent against triple-negative breast cancer.
American Psychological Association (APA)
Hsieh, Chia-Chien& Wang, Chih-Hsuan. 2018. Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1204019
Modern Language Association (MLA)
Hsieh, Chia-Chien& Wang, Chih-Hsuan. Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages. Mediators of Inflammation No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1204019
American Medical Association (AMA)
Hsieh, Chia-Chien& Wang, Chih-Hsuan. Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1204019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204019