Antioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat Hearts
Joint Authors
Souza dos Santos, Robson Augusto
Andrä, Michaela
Russ, Miriam
Jauk, Susanne
Lamacie, Mariana
Lang, Ingrid
Arnold, Robert
Brcic, Iva
Wintersteiger, Reinhold
Ortner, Astrid
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
As progressive organ shortage in cardiac transplantation demands extension of donor criteria, effort is needed to optimize graft survival.
Reactive oxygen and nitrogen species, generated during organ procurement, transplantation, and reperfusion, contribute to acute and late graft dysfunction.
The combined application of diverse substances acting via different molecular pathways appears to be a reasonable approach to face the complex mechanism of ischemia reperfusion injury.
Thus, an antioxidant solution containing α-ketoglutaric acid, 5-hydroxymethylfurfural, N-acetyl-L-methionine, and N-acetyl-selenium-L-methionine was combined with endogenous angiotensin-(1-7).
Its capacity of myocardial protection was investigated in isolated Langendorff-perfused rat hearts subjected to warm and cold ischemia.
The physiological cardiac parameters were assessed throughout the experiments.
Effects were evaluated via determination of the oxidative stress parameters malondialdehyde and carbonyl proteins as well as immunohistochemical and ultrastructural tissue analyses.
It was shown that a combination of 20% (v/v) antioxidant solution and 220 pM angiotensin-(1-7) led to the best results with a preservation of heart tissue against oxidative stress and morphological alteration.
Additionally, immediate cardiac recovery (after warm ischemia) and normal physiological performance (after cold ischemia) were recorded.
Overall, the results of this study indicate substantial cardioprotection of the novel combination with promising prospective for future clinical use.
American Psychological Association (APA)
Andrä, Michaela& Russ, Miriam& Jauk, Susanne& Lamacie, Mariana& Lang, Ingrid& Arnold, Robert…[et al.]. 2020. Antioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat Hearts. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1204058
Modern Language Association (MLA)
Andrä, Michaela…[et al.]. Antioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat Hearts. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1204058
American Medical Association (AMA)
Andrä, Michaela& Russ, Miriam& Jauk, Susanne& Lamacie, Mariana& Lang, Ingrid& Arnold, Robert…[et al.]. Antioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat Hearts. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1204058
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204058