Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease
Joint Authors
Xu, Dan
Qu, Xiaoyan
Dang, Xiaomin
Wang, Weijia
Li, Ying
Shang, Dong
Chang, Ying
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-13
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Inflammation plays a pivotal role in the pathogenesis of chronic obstructive pulmonary disease (COPD).
We evaluated the lncRNA and mRNA expression profile of peripheral blood mononuclear cells (PBMCs) from healthy nonsmokers, smokers without airflow limitation, and COPD patients.
Methods.
lncRNA and mRNA profiling of PBMCs from 17 smokers and 14 COPD subjects was detected by high-throughput microarray.
The expression of dysregulated lncRNAs was validated by qPCR.
The lncRNA targets in dysregulated mRNAs were predicted and the GO enrichment was analyzed.
The regulatory role of lncRNA ENST00000502883.1 on CXCL16 expression and consequently the effect on PBMC recruitment were investigated by siRNA knockdown and chemotaxis analysis.
Results.
We identified 158 differentially expressed lncRNAs in PBMCs from COPD subjects compared with smokers.
The dysregulated expression of 5 selected lncRNAs NR_026891.1 (FLJ10038), ENST00000502883.1 (RP11-499E18.1), HIT000648516, XR_429541.1, and ENST00000597550.1 (CTD-2245F17.3), was validated.
The GO enrichment showed that leukocyte migration, immune response, and apoptosis are the main enriched processes that previously reported to be involved in the pathogenesis of COPD.
The regulatory role of ENST00000502883.1 on CXCL16 expression and consequently the effect on PBMC recruitment was confirmed.
Conclusion.
This study may provide clues for further studies targeting lncRNAs to control inflammation in COPD.
American Psychological Association (APA)
Qu, Xiaoyan& Dang, Xiaomin& Wang, Weijia& Li, Ying& Xu, Dan& Shang, Dong…[et al.]. 2018. Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1204183
Modern Language Association (MLA)
Qu, Xiaoyan…[et al.]. Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease. Mediators of Inflammation No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1204183
American Medical Association (AMA)
Qu, Xiaoyan& Dang, Xiaomin& Wang, Weijia& Li, Ying& Xu, Dan& Shang, Dong…[et al.]. Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1204183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204183