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Fermented Wheat Germ Extract as a Redox Modulator: Alleviating Endotoxin-Triggered Oxidative Stress in Primary Cultured Rat Hepatocytes
Joint Authors
Jerzsele, Ákos
Mackei, Máté
Vörösházi, Júlia
Sebők, Csilla
Neogrády, Zsuzsanna
Mátis, Gábor
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Bioactive compounds such as benzoquinone derivates presented in fermented wheat germ extract (FWGE) have several positive effects on overall health status of humans and animals alike.
Since available data regarding the antioxidant activity of FWGE are limited, the aim of our study was to investigate its effects on the cellular redox homeostasis applying primary hepatocyte cell cultures of rat origin.
Cultures were challenged to lipopolysaccharide (LPS) treatment for 2 or 8 hours to trigger inflammatory response.
Further, culture media were concomitantly supplemented with or without FWGE (Immunovet®, 0.1% and 1%).
In order to monitor the metabolic activity of the cell cultures, CCK-8 test was applied, while reactive oxygen species (ROS) production was measured using Amplex Red method.
Malondialdehyde concentration of culture media as a specific marker of lipid peroxidation and the activity of glutathione peroxidase in cell lysates were also determined to monitor the redox status of the cultures.
Based on our findings, it can be concluded that FWGE did not show cytotoxic effects in any applied concentration in cell cultures.
Furthermore, FWGE efficiently decreased cellular ROS production and lipid peroxidation rate in case of LPS-induced inflammatory response.
However, without LPS treatment, higher concentration of FWGE increased the rate of both ROS and malondialdehyde synthesis.
This observation may refer to the prooxidant activity of high dose FWGE, which is an important beneficial effect regarding tumor cells.
However, in case of noninflamed hepatocytes, considering the results of glutathione peroxidase activity, the application of the product did not result in severe oxidative distress.
In accordance with the abovementioned findings, FWGE as a redox modulator, applied in the appropriate concentration, can serve as a promising candidate in the supplementary therapy of patients suffering from various inflammatory diseases, decreasing the free radical generation, thus avoiding the occurrence of cytotoxic effects.
American Psychological Association (APA)
Mackei, Máté& Vörösházi, Júlia& Sebők, Csilla& Neogrády, Zsuzsanna& Mátis, Gábor& Jerzsele, Ákos. 2020. Fermented Wheat Germ Extract as a Redox Modulator: Alleviating Endotoxin-Triggered Oxidative Stress in Primary Cultured Rat Hepatocytes. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1204196
Modern Language Association (MLA)
Mackei, Máté…[et al.]. Fermented Wheat Germ Extract as a Redox Modulator: Alleviating Endotoxin-Triggered Oxidative Stress in Primary Cultured Rat Hepatocytes. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1204196
American Medical Association (AMA)
Mackei, Máté& Vörösházi, Júlia& Sebők, Csilla& Neogrády, Zsuzsanna& Mátis, Gábor& Jerzsele, Ákos. Fermented Wheat Germ Extract as a Redox Modulator: Alleviating Endotoxin-Triggered Oxidative Stress in Primary Cultured Rat Hepatocytes. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1204196
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204196