The Protective Mechanism of CAY10683 on Intestinal Mucosal Barrier in Acute Liver Failure through LPSTLR4MyD88 Pathway
Joint Authors
Wang, Yao
Chen, Hui
Chen, Qian
Jiao, Fang-Zhou
Zhang, Wen-Bin
Gong, Zuo-Jiong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The purpose of this study was to investigate the protective mechanism of HDAC2 inhibitor CAY10683 on intestinal mucosal barrier in acute liver failure (ALF).
In order to establish ALF-induced intestinal epithelial barrier disruption models, D-galactosamine/LPS and LPS were, respectively, used with rats and NCM460 cell and then administrated with CAY10683.
Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells.
Real-time PCR and Western blotting were employed to detect the key mRNA and protein levels.
The intestinal epithelial tissue pathology was detected.
After interfering with CAY10683, the mRNA and protein levels of TLR4, MyD88, TRIF, and TRAF6 were decreased compared with model group (P<0.05), whereas the levels of ZO-1 and occluding were elevated (P<0.05).
The permeability was elevated in CAY10683-interfered groups, when compared with model group (P<0.05).
And the degree of intestinal epithelial tissue pathological damage in CAY10683 group was significantly reduced.
Moreover, CAY10683 significantly decreased the TLR4 staining in animal tissue.
The HDAC2 inhibitor CAY10683 could promote the damage of intestinal mucosal barrier in ALF through inhibiting LPS/TLR4/MyD88 pathway.
American Psychological Association (APA)
Wang, Yao& Chen, Hui& Chen, Qian& Jiao, Fang-Zhou& Zhang, Wen-Bin& Gong, Zuo-Jiong. 2018. The Protective Mechanism of CAY10683 on Intestinal Mucosal Barrier in Acute Liver Failure through LPSTLR4MyD88 Pathway. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1204226
Modern Language Association (MLA)
Wang, Yao…[et al.]. The Protective Mechanism of CAY10683 on Intestinal Mucosal Barrier in Acute Liver Failure through LPSTLR4MyD88 Pathway. Mediators of Inflammation No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1204226
American Medical Association (AMA)
Wang, Yao& Chen, Hui& Chen, Qian& Jiao, Fang-Zhou& Zhang, Wen-Bin& Gong, Zuo-Jiong. The Protective Mechanism of CAY10683 on Intestinal Mucosal Barrier in Acute Liver Failure through LPSTLR4MyD88 Pathway. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1204226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204226