Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro
Joint Authors
Kang, Wenyi
Huang, Jyun Yin
Xu, Zixuan
Lin, Miao-Hsia
Wang, Hui-Min David
Chen, Chung-Yi
Wang, Lin
Li, Jian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-27
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Melanoma is an aggressive cancer with high lethality.
In order to find new anticancer agents, isokotomolide A (Iso A) and secokotomolide A (Sec A) isolated from Cinnamomum kotoense were identified to be potential bioactive agents against human melanoma but without strong antioxidative properties.
Cell proliferation assay displayed Iso A and Sec A treated in the normal human skin cells showed high viabilities.
It also verified that two of them possess strong antimelanoma effect in concentration-dependent manners, especially on B16F10, A2058, MeWo, and A375 cells.
Wound healing assay presented their excellent antimigratory effects.
Through 3-N,3-N,6-N,6-N-Tetramethylacridine-3,6-diamine (acridine orange, AO) staining and Western blot, the autophagy induced by treatment was confirmed, including autophagy-related proteins (Atgs).
By using annexin V–FITC/PI double-stain, the apoptosis was confirmed, and both components also triggered the cell cycle arrest and DNA damage.
We demonstrated the correlations between the mitogen-activated protein kinase (MAPK) pathway and antimelanoma, such as caspase cascade activations.
To further evaluate in vivo experiments, the inhibition of tumor cell growth was verified through the histopathological staining in a xenograft model.
In this study, it was confirmed that Iso A and Sec A can encourage melanoma cell death via early autophagy and late apoptosis processes.
American Psychological Association (APA)
Li, Jian& Chen, Chung-Yi& Huang, Jyun Yin& Wang, Lin& Xu, Zixuan& Kang, Wenyi…[et al.]. 2020. Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1204245
Modern Language Association (MLA)
Li, Jian…[et al.]. Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1204245
American Medical Association (AMA)
Li, Jian& Chen, Chung-Yi& Huang, Jyun Yin& Wang, Lin& Xu, Zixuan& Kang, Wenyi…[et al.]. Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1204245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204245