Serotonin Exhibits Accelerated Bleomycin-Induced Pulmonary Fibrosis through TPH1 Knockout Mouse Experiments
Joint Authors
Bi, Jianbin
Liu, Chang
Feng, Yang
Li, Zeyu
Zhang, Jingyao
Cui, Ruixia
Gao, Weiman
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Pulmonary fibrosis is a chronic progressive fibrosis interstitial lung disease that is characterized by inflammatory infiltration and fibrotic changes.
5-Hydroxytryptamine (5-HT) is an important regulatory factor in inflammation, immunomodulation, and fibrosis.
The aim of this study was to investigate the role of 5-HT in bleomycin- (BLM-) induced pulmonary fibrosis through wild-type C57BL/6 (WT) and TPH1 knockout (KO) mouse experiments.
Methods.
The mice were grouped as follows: WT control group, KO control group, WT BLM group, and KO BLM group.
Mice were administrated bleomycin hydrochloride through intratracheal instillation to induce pulmonary fibrosis.
Mice were sacrificed 0, 7, 14, and 21 days after modeling, and bronchoalveolar lavage fluid (BALF) and lung tissues were collected to determine the severity of fibrotic changes.
Results.
The results showed that the weight loss of mice in the WT BLM group was more severe than that in the KO BLM group.
H&E and Sirius Red staining revealed that 5-HT markedly aggravated histological damage and fibrotic changes in the lung.
Significantly lower levels of hydroxyproline, Ashcroft fibrosis score, total BALF protein and cells, BALF tumor necrosis factor- (TNF-) α and interleukin- (IL-) 6, TNF-α and IL-6 mRNA, malondialdehyde (MDA), and myeloperoxidase- (MPO-) positive cells in the lung tissues, and fibrosis-associated proteins were discovered in the mice from the KO BLM group compared with the WT BLM group.
Conclusion.
5-HT aggravated pulmonary fibrosis mainly by promoting the inflammation, exudation of proteins and cells, oxidative stress, and upregulation of fibrosis-associated genes in the lung tissues.
American Psychological Association (APA)
Zhang, Jingyao& Cui, Ruixia& Feng, Yang& Gao, Weiman& Bi, Jianbin& Li, Zeyu…[et al.]. 2018. Serotonin Exhibits Accelerated Bleomycin-Induced Pulmonary Fibrosis through TPH1 Knockout Mouse Experiments. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1204247
Modern Language Association (MLA)
Zhang, Jingyao…[et al.]. Serotonin Exhibits Accelerated Bleomycin-Induced Pulmonary Fibrosis through TPH1 Knockout Mouse Experiments. Mediators of Inflammation No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1204247
American Medical Association (AMA)
Zhang, Jingyao& Cui, Ruixia& Feng, Yang& Gao, Weiman& Bi, Jianbin& Li, Zeyu…[et al.]. Serotonin Exhibits Accelerated Bleomycin-Induced Pulmonary Fibrosis through TPH1 Knockout Mouse Experiments. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1204247
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204247