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Superoxide Dismutase Mimic, MnTE-2-PyP Enhances Rectal Anastomotic Strength in Rats after Preoperative Chemoradiotherapy
Joint Authors
Tong, Qiang
Jiang, Yue
Tong, Shilun
Wang, Zhihua
Zhou, Rui
Yang, Yu
Wang, Qi
Luo, Jiajun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Rectal cancer is one of the malignant diseases with high morbidity and mortality in the world.
Currently, surgical resection is the main treatment method, and preoperative chemoradiotherapy (CRT) is widely used in clinical application to increase resectability and decrease the local recurrence rate.
However, CRT increases the risk of colon anastomotic leak, and currently, there are no FDA approved treatments against this side effect.
It is essential to develop new drugs to reduce postoperative anastomotic leak after preoperative CRT.
Methods.
90 rats underwent standard resection and intestine anastomosis treatment and were divided into six groups for different treatments.
During the relaparotomy, bursting pressure of anastomosis was measured and intestinal segments were taken for histopathologic examination and biochemical analyses.
RT-PCR and ELISA were applied to measure matrix metalloproteinase (MMP) mRNA and protein levels.
Blood vessels were observed by immunohistochemistry, and collagen deposition was observed by Picrosirius Red staining.
Results.
Preoperative CRT reduced the postoperative anastomotic strength.
MnTE-2-PyP increased the bursting pressure and hydroxyproline levels of intestine anastomosis after CRT treatment.
Mechanically, MnTE-2-PyP decreased the MMP levels and increased microvessel density (MVD) and collagen deposition.
The MMP inhibitor doxycycline had a positive effect on anastomosis healing, but was inferior to MnTE-2-PyP.
Conclusions.
MnTE-2-PyP enhanced intestine anastomotic strength in rats with preoperative CRT.
Specifically, MnTE-2-PyP decreased MMP levels and increased MVD in anastomosis.
Therefore, MnTE-2-PyP may be helpful in the prevention of anastomotic leak after preoperative CRT.
American Psychological Association (APA)
Yang, Yu& Wang, Qi& Luo, Jiajun& Jiang, Yue& Zhou, Rui& Tong, Shilun…[et al.]. 2020. Superoxide Dismutase Mimic, MnTE-2-PyP Enhances Rectal Anastomotic Strength in Rats after Preoperative Chemoradiotherapy. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1204273
Modern Language Association (MLA)
Yang, Yu…[et al.]. Superoxide Dismutase Mimic, MnTE-2-PyP Enhances Rectal Anastomotic Strength in Rats after Preoperative Chemoradiotherapy. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1204273
American Medical Association (AMA)
Yang, Yu& Wang, Qi& Luo, Jiajun& Jiang, Yue& Zhou, Rui& Tong, Shilun…[et al.]. Superoxide Dismutase Mimic, MnTE-2-PyP Enhances Rectal Anastomotic Strength in Rats after Preoperative Chemoradiotherapy. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1204273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204273