The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans
Joint Authors
Zhou, Xiao-Gang
Wu, Gui-Sheng
Wan, Qin-Li
Yang, Zhong-Lin
Ding, Ai-Jun
Pu, Yuan-Zhu
Luo, Huai-Rong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Aging is a complex life process, and a unified view is that metabolism plays key roles in all biological processes.
Here, we determined the lipidomic profile of Caenorhabditis elegans (C.
elegans) using ultraperformance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS).
Using a nontargeted approach, we detected approximately 3000 species.
Analysis of the lipid metabolic profiles at young adult and ten-day-old ages among wild-type N2, glp-1 defective mutant, and double mutant daf-16;glp-1 uncovered significant age-related differences in the total amount of phosphatidylcholines (PC), sphingomyelins (SM), ceramides (Cer), diglycerides (DG), and triglycerides (TG).
In addition, the age-associated lipid profiles were characterized by ratio of polyunsaturated (PUFA) over monounsaturated (MUFA) lipid species.
Lipid metabolism modulation plays an important role in reproduction-regulated aging; to identify the variations of lipid metabolites during germ line loss-induced longevity, we investigated the lipidomic profiles of long-lived glp-1/notch receptor mutants, which have reproductive deficiency when grown at nonpermissive temperature.
The results showed that there was some age-related lipid variation, including TG 40:2, TG 40:1, and TG 41:1, which contributed to the long-life phenotype.
The longevity of glp-1 mutant was daf-16-dependent; the lipidome analysis of daf-16;glp-1 double mutant revealed that the changes of some metabolites in the glp-1 mutant were daf-16-dependent, while other metabolites displayed more complex epistatic patterns.
We first conducted a comprehensive lipidome analysis to provide novel insights into the relationships between longevity and lipid metabolism regulated by germ line signals in C.
elegans.
American Psychological Association (APA)
Wan, Qin-Li& Yang, Zhong-Lin& Zhou, Xiao-Gang& Ding, Ai-Jun& Pu, Yuan-Zhu& Luo, Huai-Rong…[et al.]. 2019. The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204275
Modern Language Association (MLA)
Wan, Qin-Li…[et al.]. The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204275
American Medical Association (AMA)
Wan, Qin-Li& Yang, Zhong-Lin& Zhou, Xiao-Gang& Ding, Ai-Jun& Pu, Yuan-Zhu& Luo, Huai-Rong…[et al.]. The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204275
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204275