Dicalcium Silicate Induced Proinflammatory Responses through TLR2-Mediated NF-κB and JNK Pathways in the Murine RAW 264.7 Macrophage Cell Line
Joint Authors
Chen, Liangjiao
Lai, Shixiang
Cao, Wei
Cui, Shiman
Li, Xingyang
Zhong, Wenchao
Ma, Mingyu
Zhang, Qingbin
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-02
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Proinflammatory responses are important aspects of the immune response to biomaterials, which may cause peri-implantitis and implant shedding.
The purpose of this study was to test the cytotoxicity and proinflammatory effects of dicalcium silicate particles on RAW 264.7 macrophages and to investigate the proinflammatory response mechanism induced by C2S and tricalcium phosphate (TCP).
C2S and TCP particles were characterized using scanning electron microscopy (SEM), energy spectrum analysis (EDS) and X-ray diffraction (XRD).
Cytotoxicity and apoptosis assays with C2S and TCP in the murine RAW 264.7 cell line were tested using the cell counting kit-8 (CCK-8) assay and flow cytometry (FCM).
The detection results showed that C2S and TCP particles had no obvious toxicity in RAW 264.7 cells and did not cause obvious apoptosis, although they both caused an oxidative stress response by producing ROS when the concentrations were at 100 μg/mL.
C2S particles are likely to induce a proinflammatory response by inducing high TLR2, TNF-α mRNA, TNF-α proinflammatory cytokine, p-IκB, and p-JNK1 + JNK2 + JNK3 expression levels.
When we added siRNA-TLR2-1, a significant reduction was observed.
These findings support the theory that C2S particles induce proinflammatory responses through the TLR2-mediated NF-κB and JNK pathways in the murine RAW 264.7 macrophage cell line.
American Psychological Association (APA)
Lai, Shixiang& Chen, Liangjiao& Cao, Wei& Cui, Shiman& Li, Xingyang& Zhong, Wenchao…[et al.]. 2018. Dicalcium Silicate Induced Proinflammatory Responses through TLR2-Mediated NF-κB and JNK Pathways in the Murine RAW 264.7 Macrophage Cell Line. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1204278
Modern Language Association (MLA)
Lai, Shixiang…[et al.]. Dicalcium Silicate Induced Proinflammatory Responses through TLR2-Mediated NF-κB and JNK Pathways in the Murine RAW 264.7 Macrophage Cell Line. Mediators of Inflammation No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1204278
American Medical Association (AMA)
Lai, Shixiang& Chen, Liangjiao& Cao, Wei& Cui, Shiman& Li, Xingyang& Zhong, Wenchao…[et al.]. Dicalcium Silicate Induced Proinflammatory Responses through TLR2-Mediated NF-κB and JNK Pathways in the Murine RAW 264.7 Macrophage Cell Line. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1204278
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204278