Cholesterol Prevents Hypoxia-Induced Hypoglycemia by Regulation of a Metabolic Ketogenic Shift
Joint Authors
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Blood cholesterol levels have been connected to high-altitude adaptation.
In the present study, we treated mice with high-cholesterol diets following exposure to acute hypoxic stress and evaluated the effects of the diets on whole-body, liver glucose, and liver fat metabolism.
For rapid cholesterol liver uptake, 6-week-old male C57BL/J6 mice were fed with high-cholesterol/cholic acid (CH) diet for 6 weeks and then were exposed to gradual oxygen level reduction for 1 h and hypoxia at 7% oxygen for additional 1 hour using a hypoxic chamber.
Animals were than sacrificed, and metabolic markers were evaluated.
Hypoxic treatment had a strong hypoglycemic effect that was completely blunted by CH treatment.
Decreases in gluconeogenesis and glycogenolysis as well as an increase in ketone body formation were observed.
Such changes indicate a metabolic shift from glucose to fat utilization due to activation of the inducible nitric oxide synthase/AMPK axis in the CH-treated animals.
Increased ketogenesis was also observed in vitro in hepatocytes after cholesterol treatment.
In conclusion, our results show for the first time that cholesterol contributes to metabolic shift and adaptation to hypoxia in vivo and in vitro through induction of HIF-1α and iNOS expression.
American Psychological Association (APA)
Miron, Naama& Tirosh, Oren. 2019. Cholesterol Prevents Hypoxia-Induced Hypoglycemia by Regulation of a Metabolic Ketogenic Shift. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204321
Modern Language Association (MLA)
Miron, Naama& Tirosh, Oren. Cholesterol Prevents Hypoxia-Induced Hypoglycemia by Regulation of a Metabolic Ketogenic Shift. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1204321
American Medical Association (AMA)
Miron, Naama& Tirosh, Oren. Cholesterol Prevents Hypoxia-Induced Hypoglycemia by Regulation of a Metabolic Ketogenic Shift. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204321
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204321