Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed by Escherichia coli Lipopolysaccharides
Joint Authors
Kabanov, Dmitry S.
Vwedenskaya, Olga Yu.
Fokina, Marina A.
Morozova, Elena M.
Grachev, Sergey V.
Prokhorenko, Isabella R.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Lipopolysaccharides (LPS) from Gram-negative bacteria prime human polymorphonuclear neutrophils (PMNs) via multicomponent receptor cluster including CD14 and MD-2·TLR4 for the enhanced release of reactive oxygen species (ROS) were triggered by bacterial derived peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP).
In this study, we investigated the impact of CD14 on LPS-induced priming of human PMNs for fMLP-triggered ROS generation (respiratory or oxidative) burst.
Monoclonal antibodies against human CD14 (mAbs) as well as isotype-matched IgG2a did not influence significantly fMLP-triggered ROS production from LPS-unprimed PMNs.
Anti-CD14 mAbs (clone UCHM-1) attenuated LPS-induced priming of PMNs as it had been mirrored by fMLP-triggered decrease of ROS production.
Similar priming activity of S-LPS or Re-LPS from Escherichia coli for fMLP-triggered ROS release from PMNs was found.
Obtained results suggest that glycosylphosphatidylinositol-anchored CD14 is the key player in LPS-induced PMN priming for fMLP-triggered ROS production.
We believe that blockade of CD14 on the cell surface and clinical use of anti-CD14 mAbs or their Fab fragments may diminish the production of ROS and improve outcomes during cardiovascular diseases manifested by LPS-induced inflammation.
American Psychological Association (APA)
Kabanov, Dmitry S.& Vwedenskaya, Olga Yu.& Fokina, Marina A.& Morozova, Elena M.& Grachev, Sergey V.& Prokhorenko, Isabella R.. 2019. Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed by Escherichia coli Lipopolysaccharides. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1204420
Modern Language Association (MLA)
Kabanov, Dmitry S.…[et al.]. Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed by Escherichia coli Lipopolysaccharides. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1204420
American Medical Association (AMA)
Kabanov, Dmitry S.& Vwedenskaya, Olga Yu.& Fokina, Marina A.& Morozova, Elena M.& Grachev, Sergey V.& Prokhorenko, Isabella R.. Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed by Escherichia coli Lipopolysaccharides. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1204420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204420