Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
Joint Authors
Khatri, Neeraj
Chopra, Bhupinder Singh
Ashish, Renu
Vaid, Bhavna
Raut, Sachin
Sagar, Amin
Badmalia, Maulik D.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Delineation of factors which affect wound healing would be of immense value to enable on-time or early healing and reduce comorbidities associated with infections or biochemical stress like diabetes.
Plasma gelsolin has been identified earlier to significantly enable injury recovery compared to placebo.
This study evaluates the role of rhuGSN for its antioxidant and wound healing properties in murine fibroblasts (3T3-L1 cell line).
Total antioxidant capacity of rhuGSN increased in a concentration-dependent manner (0.75-200 μg/mL).
Cells pretreated with 0.375 and 0.75 μg/mL rhuGSN for 24 h exhibited a significant increase in viability in a MTT assay.
Preincubation of cells with rhuGSN for 24 h followed by oxidative stress induced by exposure to H2O2 for 3 h showed cytoprotective effect.
rhuGSN at 12.5 and 25 μg/mL concentration showed an enhanced cell migration after 20 h of injury in a scratch wound healing assay.
The proinflammatory cytokine IL-6 levels were elevated in the culture supernatant.
These results establish an effective role of rhuGSN against oxidative stress induced by H2O2 and in wound healing of 3T3-L1 fibroblast cells.
American Psychological Association (APA)
Vaid, Bhavna& Chopra, Bhupinder Singh& Raut, Sachin& Sagar, Amin& Badmalia, Maulik D.& Ashish, Renu…[et al.]. 2020. Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1204422
Modern Language Association (MLA)
Vaid, Bhavna…[et al.]. Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1204422
American Medical Association (AMA)
Vaid, Bhavna& Chopra, Bhupinder Singh& Raut, Sachin& Sagar, Amin& Badmalia, Maulik D.& Ashish, Renu…[et al.]. Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1204422
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204422