Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo
Joint Authors
Deng, Mingwu
Xu, Yuda
Yu, Ziyou
Wang, Xiangsheng
Cai, Yizuo
Zheng, Hongjie
Li, Wei
Zhang, Wenjie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Nanofat can protect against ultraviolet B- (UVB-) induced damage in nude mice.
Fat extract (FE) is a cell-free fraction isolated from nanofat that is enriched with a variety of growth factors.
Objective.
To determine whether FE can protect against UVB-induced photoaging in cultured dermal fibroblasts and in nude mice.
Method.
For the in vitro study, human dermal skin fibroblasts were pretreated with FE 24 h prior to UVB irradiation.
Generation of reactive oxygen species (ROS) was analyzed immediately following irradiation, while cell cycle analysis was performed 24 h after UVB irradiation.
Senescence-associated β-galactosidase (SA-β-gal) expression, cell proliferation, and expression of glutathione peroxidase 1 (GPX-1), catalase, superoxide dismutase-1 (SOD-1), SOD-2, and collagen type 1 (COL-1) were investigated 72 h after UVB irradiation.
For the in vivo study, the dorsal skin of nude mice was irradiated with UVB and mice were then treated with FE for 8 weeks.
The thickness of the dermis, capillary density, and apoptotic cells in skin tissue sections were investigated after treatment.
The expression of GPX-1, catalase, SOD-2, SOD-1, and COL-1 in the tissue was also measured.
Result.
FE significantly increased cell proliferation and protected cells against UVB-induced cell death and cell cycle arrest.
FE reduced ROS and the number of aged cells induced by UVB irradiation.
FE promoted the expression of COL-1 and GPX-1 in cultured dermal fibroblasts.
FE treatment of UVB-irradiated skin increased dermal thickness and capillary density, decreased the number of apoptotic cells, and promoted the expression of COL-1 and GPX-1.
Conclusion.
FE protects human dermal fibroblasts and the skin of nude mice from UVB-induced photoaging through its antioxidant, antiapoptotic, and proangiogenic activities.
American Psychological Association (APA)
Deng, Mingwu& Xu, Yuda& Yu, Ziyou& Wang, Xiangsheng& Cai, Yizuo& Zheng, Hongjie…[et al.]. 2019. Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204463
Modern Language Association (MLA)
Deng, Mingwu…[et al.]. Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1204463
American Medical Association (AMA)
Deng, Mingwu& Xu, Yuda& Yu, Ziyou& Wang, Xiangsheng& Cai, Yizuo& Zheng, Hongjie…[et al.]. Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204463