Interplay between Nox2 Activity and Platelet Activation in Patients with Sepsis and Septic Shock: A Prospective Study
Joint Authors
Frati, Giacomo
Miraldi, Fabio
Peruzzi, Mariangela
Nocella, Cristina
Sciarretta, Sebastiano
Tiseo, Giusy
Cavarretta, Elena
Forniti, Arianna
Spagnolello, Ornella
Baldini, Enrico
Bertazzoni, Giuliano
Menichetti, Francesco
Marullo, Antonino G. M.
Morelli, Andrea
Carnevale, Roberto
Falcone, Marco
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Although preclinical studies highlighted the potential role of NADPH oxidase (NOX) in sepsis, only few studies evaluated the oxidative stress in patients with sepsis and septic shock.
The objective of the study is to appraise the oxidative stress status and platelet function in patients with sepsis and septic shock compared to healthy controls.
Methods and Results.
Patients with sepsis or septic shock admitted to the hospital Policlinico Umberto I (Sapienza University, Rome) underwent a blood sample collection within 1 hour from admission.
Platelet aggregation, serum thromboxane B2 (TxB2), soluble NOX2-derived peptides (sNox2-dp), and hydrogen peroxide breakdown activity (HBA) were measured and compared to those of healthy volunteers.
Overall, 33 patients were enrolled; of these, 20 (60.6%) had sepsis and 13 (39.4%) septic shock.
Compared to healthy controls (n=10, age 67.8±3.2, male 50%), patients with sepsis and septic shock had higher platelet aggregation (49% (IQR 45-55), 60% (55.75-67.25), and 73% (IQR 69-80), respectively, p<0.001), higher serum TxB2 (77.5 (56.5-86.25), 122.5 (114-131.5), and 210 (195-230) pmol/L, respectively, p<0.001), higher sNox2-dp (10 (7.75-12), 19.5 (17.25-21), and 33 (29.5-39) pg/mL, respectively, p<0.001), and lower HBA (75% (67.25-81.5), 50% (45-54.75), and 27% (21.5-32.5), respectively, p<0.001).
Although not statistically significant, a trend in higher levels of serum TxB2 and sNox2-dp in patients who died was observed.
Conclusions.
Patients with septic shock exhibit higher Nox2 activity and platelet activation than patients with sepsis.
These insights joined to better knowledge of these mechanisms could guide the identification of future prognostic biomarkers and new therapeutic strategies in the scenario of septic shock.
American Psychological Association (APA)
Marullo, Antonino G. M.& Miraldi, Fabio& Morelli, Andrea& Frati, Giacomo& Carnevale, Roberto& Falcone, Marco…[et al.]. 2020. Interplay between Nox2 Activity and Platelet Activation in Patients with Sepsis and Septic Shock: A Prospective Study. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1204470
Modern Language Association (MLA)
Marullo, Antonino G. M.…[et al.]. Interplay between Nox2 Activity and Platelet Activation in Patients with Sepsis and Septic Shock: A Prospective Study. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1204470
American Medical Association (AMA)
Marullo, Antonino G. M.& Miraldi, Fabio& Morelli, Andrea& Frati, Giacomo& Carnevale, Roberto& Falcone, Marco…[et al.]. Interplay between Nox2 Activity and Platelet Activation in Patients with Sepsis and Septic Shock: A Prospective Study. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1204470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204470