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Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet
Joint Authors
Jo, Ara
Joh, Hea Min
Chung, Tae Hun
Chung, Jin Woong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-31
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Cold atmospheric plasma (CAP) has been reported to have strong anticancer effects in vitro and in vivo.
CAP has been known to induce apoptosis in most cancer cells by treatment to cells using direct and indirect treatment methods.
There are many reports of apoptosis pathways induced by CAP, but for indirect treatment, there is still a lack of fundamental research on how CAP can cause apoptosis in cancer cells.
In this study, we applied an indirect treatment method to determine how CAP can induce cancer cell death.
First, plasma-activated medium (PAM) was produced by a 2.45 GHz microwave-excited atmospheric pressure plasma jet (ME-APPJ).
Next, the amounts of various reactive species in the PAM were estimated using colorimetric methods.
The concentration of NO2– and H2O2 in PAM cultured with cancer cells was measured, and intracellular reactive oxidative stress (ROS) changes were observed using flow cytometry.
When PAM was incubated with A549 lung cancer cells, there was little change in NO2– concentration, but the concentration of H2O2 gradually decreased after 30 min.
While the intracellular ROS of A549 cells was rapidly increased at 2 hours, there was no significant change in that of PAM-treated normal cells.
Furthermore, PAM had a significant cytotoxic effect on A549 cells but had little effect on normal cell viability.
In addition, using flow cytometry, we confirmed that apoptosis of A549 cells occurred following flow cytometry and western blot analysis.
These results suggest that among various reactive species produced by PAM, hydrogen peroxide plays a key role in inducing cancer cell apoptosis.
American Psychological Association (APA)
Jo, Ara& Joh, Hea Min& Chung, Tae Hun& Chung, Jin Woong. 2020. Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1204484
Modern Language Association (MLA)
Jo, Ara…[et al.]. Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1204484
American Medical Association (AMA)
Jo, Ara& Joh, Hea Min& Chung, Tae Hun& Chung, Jin Woong. Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1204484
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204484