![](/images/graphics-bg.png)
Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck
Joint Authors
Vigo, Daniele
Mattioli, Simona
Brecchia, Gabriele
Menchetti, Laura
Barbato, Olimpia
Sforna, Monica
Curone, Giulio
Tecilla, Marco
Riva, Federica
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Infections of the genital tract can perturb the fertility in humans and animals.
Pathogen recognition and activation of innate immunity onset through the pattern recognition receptor activation, such as Toll-like receptor 4 (TLR4), leading to the production of proinflammatory cytokines and mediators.
TLR4 is expressed both on leukocytes and nonimmune cells.
Rabbit TLR4 shows great similarity to its human counterpart.
Moreover, the TLR4 signalling pathway could be modulated by long-chain polyunsaturated fatty acids (LC-PUFA).
The objectives of this study were (i) to determine the expression levels of TLR4 and proinflammatory cytokines in the reproductive hypothalamic-gonadal axis of the male rabbit and (ii) to evaluate if the n-3 PUFA-enriched diets can modify their expression levels in the tissues and LC-PUFA profiles in seminal plasma.
Fifteen rabbit bucks (n=5/experimental group) were fed with different diets: commercial standard (group C), rich in extruded linseed (10%, group L), and in fish oil (3%, group FO) for 110 days.
TLR4, TNF-α, and IL-1β mRNA were ubiquitously expressed throughout the hypothalamic-gonadal axis.
However, TLR4 mRNA expression was lower in the hypothalamus than the epididymis (P<0.01), seminal vesicles (P<0.01), and pituitary gland (P<0.05).
Dietary enrichment in PUFA did not modify the gene expression profile nor the histological characteristics of the tissues.
Conversely in seminal plasma, rabbits fed with L and FO had lower n-6 (P<0.05), LC-PUFA n-6 (P<0.05), and n-6/n-3 ratio (P<0.05) but higher n-3 (P<0.001) and LC-PUFA n-3 (P<0.01) compared to the control group.
Our study builds a map of the gene expression of TRL4 and proinflammatory cytokines in the reproductive hypothalamic-gonadal axis of the male rabbit, fundamental step for understanding the immune defence mechanisms.
Diets enriched in LC-PUFA did not affect basal gene expression but modulated sperm fatty acid composition.
Finally, rabbit may be an excellent animal model to study the relationship between inflammation and infertility, and the nutritional modulation of immune functions.
American Psychological Association (APA)
Menchetti, Laura& Barbato, Olimpia& Sforna, Monica& Vigo, Daniele& Mattioli, Simona& Curone, Giulio…[et al.]. 2020. Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1204516
Modern Language Association (MLA)
Menchetti, Laura…[et al.]. Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1204516
American Medical Association (AMA)
Menchetti, Laura& Barbato, Olimpia& Sforna, Monica& Vigo, Daniele& Mattioli, Simona& Curone, Giulio…[et al.]. Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1204516
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204516