Safflor Yellow B Attenuates Ischemic Brain Injury via Downregulation of Long Noncoding AK046177 and Inhibition of MicroRNA-134 Expression in Rats
Joint Authors
Wang, Chaoyun
Wan, Hongzhi
Wang, Qiaoyun
Sun, Hongliu
Sun, Yeying
Wang, Kexin
Zhang, Chunxiang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-04
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Stroke breaks the oxidative balance in the body and causes extra reactive oxygen species (ROS) generation, leading to oxidative stress damage.
Long noncoding RNAs (lncRNAs) and microRNAs play pivotal roles in oxidative stress-mediated brain injury.
Safflor yellow B (SYB) was able to effectively reduce ischemia-mediated brain damage by increasing antioxidant capacity and inhibiting cell apoptosis.
In this study, we investigated the putative involvement of lncRNA AK046177 and microRNA-134 (miR-134) regulation in SYB against ischemia/reperfusion- (I/R-) induced neuronal injury.
I/R and oxygen-glucose deprivation/reoxygenation (OGD/R) were established in vivo and in vitro.
Cerebral infarct volume, neuronal apoptosis, and protein expression were detected.
The effects of SYB on cell activity, cell respiration, nuclear factor erythroid 2-related factor 2 (Nrf2), antioxidant enzymes, and ROS were evaluated.
I/R or OGD/R upregulated the expression of AK046177 and miR-134 and subsequently inhibited the activation and expression of CREB, which caused ROS generation and brain/cell injury.
SYB attenuated the effects of AK046177, inhibited miR-134 expression, and promoted CREB activation, which in turn promoted Nrf2 expression, and then increased antioxidant capacities, improved cell respiration, and reduced apoptosis.
We suggested that the antioxidant effects of SYB were driven by an AK046177/miR-134/CREB-dependent mechanism that inhibited this pathway, and that SYB has potential use in reducing or possibly preventing I/R-induced neuronal injury.
American Psychological Association (APA)
Wang, Chaoyun& Wan, Hongzhi& Wang, Qiaoyun& Sun, Hongliu& Sun, Yeying& Wang, Kexin…[et al.]. 2020. Safflor Yellow B Attenuates Ischemic Brain Injury via Downregulation of Long Noncoding AK046177 and Inhibition of MicroRNA-134 Expression in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1204553
Modern Language Association (MLA)
Wang, Chaoyun…[et al.]. Safflor Yellow B Attenuates Ischemic Brain Injury via Downregulation of Long Noncoding AK046177 and Inhibition of MicroRNA-134 Expression in Rats. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-20.
https://search.emarefa.net/detail/BIM-1204553
American Medical Association (AMA)
Wang, Chaoyun& Wan, Hongzhi& Wang, Qiaoyun& Sun, Hongliu& Sun, Yeying& Wang, Kexin…[et al.]. Safflor Yellow B Attenuates Ischemic Brain Injury via Downregulation of Long Noncoding AK046177 and Inhibition of MicroRNA-134 Expression in Rats. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1204553
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204553