4-Phenylbutyric Acid Reduces Endoplasmic Reticulum Stress in Chondrocytes That Is Caused by Loss of the Protein Disulfide Isomerase ERp57

Joint Authors

Rellmann, Yvonne
Gronau, Isabel
Dreier, Rita
Hansen, U.

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-10-29

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Biology

Abstract EN

Objective.

The integrity of cartilage depends on the correct synthesis of extracellular matrix (ECM) components.

In case of insufficient folding of proteins in the endoplasmic reticulum (ER) of chondrocytes, ECM proteins aggregate, ER stress evolves, and the unfolded protein response (UPR) is initiated.

By this mechanism, chondrocytes relieve the stress condition or initiate cell death by apoptosis.

Especially persistent ER stress has emerged as a pathogenic mechanism in cartilage diseases, such as chondrodysplasias and osteoarthritis.

As pharmacological intervention is not available yet, it is of great interest to understand cartilage ER stress in detail and to develop therapeutics to intervene.

Methods.

ERp57-deficient chondrocytes were generated by CRISPR/Cas9-induced KO.

ER stress and autophagy were studied on mRNA and protein level as well as by transmission electron microscopy (TEM) in chondrocyte micromass or cartilage explant cultures of ERp57 KO mice.

Thapsigargin (Tg), an inhibitor of the ER-residing Ca2+-ATPase, and 4-Phenylbutyric acid (4-PBA), a small molecular chemical chaperone, were applied to induce or inhibit ER stress.

Results.

Our data reveal that the loss of the protein disulfide isomerase ERp57 is sufficient to induce ER stress in chondrocytes.

4-PBA efficiently diffuses into cartilage explant cultures and diminishes excessive ER stress in chondrocytes dose dependently, no matter if it is induced by ERp57 KO or stimulation with Tg.

Conclusion.

ER-stress-related diseases have different sources; therefore, various targets for therapeutic treatment exist.

In the future, 4-PBA may be used alone or in combination with other drugs for the treatment of ER-stress-related skeletal disorders in patients.

American Psychological Association (APA)

Rellmann, Yvonne& Gronau, Isabel& Hansen, U.& Dreier, Rita. 2019. 4-Phenylbutyric Acid Reduces Endoplasmic Reticulum Stress in Chondrocytes That Is Caused by Loss of the Protein Disulfide Isomerase ERp57. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1204598

Modern Language Association (MLA)

Rellmann, Yvonne…[et al.]. 4-Phenylbutyric Acid Reduces Endoplasmic Reticulum Stress in Chondrocytes That Is Caused by Loss of the Protein Disulfide Isomerase ERp57. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1204598

American Medical Association (AMA)

Rellmann, Yvonne& Gronau, Isabel& Hansen, U.& Dreier, Rita. 4-Phenylbutyric Acid Reduces Endoplasmic Reticulum Stress in Chondrocytes That Is Caused by Loss of the Protein Disulfide Isomerase ERp57. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1204598

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1204598