![](/images/graphics-bg.png)
Epigallocatechin Gallate Effectively Affects Senescence and Anti-SASP via SIRT3 in 3T3-L1 Preadipocytes in Comparison with Other Bioactive Substances
Joint Authors
Lilja, Stephanie
Oldenburg, Julia
Pointner, Angelika
Dewald, Laura
Lerch, Mariam
Hippe, Berit
Switzeny, Olivier
Haslberger, Alexander
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Aim.
We investigated different bioactive compounds including epigallocatechin gallate (EGCG), anthocyanidin, resveratrol, phloretin, spermidine, butyrate, and β-hydroxybutyrate with regard to their effect on SIRT3 via NRF2 and modulation of the proinflammatory senescence-associated secretory phenotype (SASP) in senescence induced 3T3-L1 preadipocytes.
Methods.
For induction of senescence, 3T3-L1 preadipocytes were incubated with bromodeoxyuridine (BrdU) for 8 days.
Cell cycle inhibition was observed, and β-galactosidase activity was measured.
After BrdU treatment, cells were treated with different bioactive compounds in various concentrations for 96 h.
ELISA was used for determining proinflammatory cytokine IL6 in SASP cells.
Results.
CDKN1a increased significantly after BrdU incubation compared to untreated control (p<0.01).
All secondary plant ingredients used for treatment, but not anthocyanidin 50 μM, decrease CDKN1a expression (p<0.05), whereas most endogenous substances did not attenuate CDKN1a.
IL6 secretion positively correlated with CDKN1a (p<0.01), whereas EGCG could diminish both, IL6 and CDKN1a with the strongest effect (p<0.01).
Although NRF2 positively correlated with SIRT3 activation (p<0.05), only resveratrol (p<0.01) and anthocyanidin (p<0.05) could activate NRF2 significantly.
Solely anthocyanidin 50 μM (p<0.05) and 100 μM (p<0.01) and EGCG 50 μM (p<0.01) could increase SIRT3 expression.
Activation of SIRT3 with EGCG correlated with lowered IL6 secretion significantly (p<0.05) but not with anthocyanidin.
Conclusion.
Accumulation of senescent cells in adipose tissue plays an important role in obesity and age-related diseases.
SIRT3, located in the mitochondria, can regulate ROS via different pathways.
Thus, targeting SIRT3 activating compounds such as EGCG may delay senescence of cells and senescence induced inflammatory processes.
American Psychological Association (APA)
Lilja, Stephanie& Oldenburg, Julia& Pointner, Angelika& Dewald, Laura& Lerch, Mariam& Hippe, Berit…[et al.]. 2020. Epigallocatechin Gallate Effectively Affects Senescence and Anti-SASP via SIRT3 in 3T3-L1 Preadipocytes in Comparison with Other Bioactive Substances. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204617
Modern Language Association (MLA)
Lilja, Stephanie…[et al.]. Epigallocatechin Gallate Effectively Affects Senescence and Anti-SASP via SIRT3 in 3T3-L1 Preadipocytes in Comparison with Other Bioactive Substances. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1204617
American Medical Association (AMA)
Lilja, Stephanie& Oldenburg, Julia& Pointner, Angelika& Dewald, Laura& Lerch, Mariam& Hippe, Berit…[et al.]. Epigallocatechin Gallate Effectively Affects Senescence and Anti-SASP via SIRT3 in 3T3-L1 Preadipocytes in Comparison with Other Bioactive Substances. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204617