Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer
Joint Authors
Pariz, Juliana R.
Ranéa, Caroline
Monteiro, Rosa A. C.
Evenson, Donald P.
Hallak, Jorge
Drevet, J. R.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics.
Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process.
This study evaluates the protective and stimulant effect of melatonin and caffeine supplementation on the functional characteristics of human spermatozoa before and after freezing.
Thirty seminal samples from normozoospermic men aged 19–45 years old collected between October 2012 and May 2017 were included.
Semen samples were supplemented with either 2 mM melatonin (MEL) prior to cryopreservation, 2 mM caffeine (CAF) in postthaw, or CAF and MEL (CM) in precryopreservation and postthaw, respectively.
Kinetics and seminal parameters, mitochondrial activity, DNA fragmentation, and reactive oxygen species (ROS) levels were analyzed before and after cryopreservation.
A significant reduction in sperm concentration, total and progressive motility, sperm kinetics, and mitochondrial activity, as well as a significant increase in DNA fragmentation and ROS production in postthaw samples compared to fresh samples, was identified.
After administration of a caffeine and/or melatonin supplement, there was a significant increase in progressive motility in the CAF (p=0.005) and CM (p=0.048) groups, as well as mitochondrial activity in the CM group (p<0.05).
Cryopreservation has negative effects on overall sperm quality and increases ROS production.
A combination of caffeine and melatonin in prefreeze and postthaw sperm samples has proven to be a very effective and simple way to improve semen quality.
This will be particularly useful for initial low-quality semen samples, those which suffer the most from the freezing/thawing process.
American Psychological Association (APA)
Pariz, Juliana R.& Ranéa, Caroline& Monteiro, Rosa A. C.& Evenson, Donald P.& Drevet, J. R.& Hallak, Jorge. 2019. Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1204637
Modern Language Association (MLA)
Pariz, Juliana R.…[et al.]. Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1204637
American Medical Association (AMA)
Pariz, Juliana R.& Ranéa, Caroline& Monteiro, Rosa A. C.& Evenson, Donald P.& Drevet, J. R.& Hallak, Jorge. Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1204637
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204637