Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle Type
Joint Authors
Grune, Tilman
Fernando, Raquel
Castro, José Pedro
Flore, Tanina
Deubel, Stefanie
Ott, Christiane
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The skeletal muscle plays an important role in maintaining whole-body mechanics, metabolic homeostasis, and interorgan crosstalk.
However, during aging, functional and structural changes such as fiber integrity loss and atrophy can occur across different species.
A commonly observed hallmark of aged skeletal muscle is the accumulation of oxidatively modified proteins and protein aggregates which point to an imbalance in proteostasis systems such as degradation machineries.
Recently, we showed that the ubiquitin-proteasomal system was impaired.
Specifically, the proteasomal activity, which was declining in aged M.
soleus (SOL) and M.
extensor digitorum longus (EDL).
Therefore, in order to understand whether another proteolytic system would compensate the decline in proteasomal activity, we aimed to investigate age-related changes in the autophagy-lysosomal system (ALS) in SOL, mostly consisting of slow-twitch fibers, and EDL, mainly composed of fast-twitch fibers, from young (4 months) and old (25 months) C57BL/6JRj mice.
Here, we focused on changes in the content of modified proteins and the ALS.
Our results show that aged SOL and EDL display high levels of protein modifications, particularly in old SOL.
While autophagy machinery appears to be functional, lysosomal activity declines gradually in aged SOL.
In contrast, in old EDL, the ALS seems to be affected, demonstrated by an increased level of key autophagy-related proteins, which are known to accumulate when their delivery or degradation is impaired.
In fact, lysosomal activity was significantly decreased in old EDL.
Results presented herein suggest that the ALS can compensate the high levels of modified proteins in the more oxidative muscle, SOL, while EDL seems to be more prone to ALS age-related alterations.
American Psychological Association (APA)
Fernando, Raquel& Castro, José Pedro& Flore, Tanina& Deubel, Stefanie& Grune, Tilman& Ott, Christiane. 2020. Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle Type. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1204643
Modern Language Association (MLA)
Fernando, Raquel…[et al.]. Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle Type. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1204643
American Medical Association (AMA)
Fernando, Raquel& Castro, José Pedro& Flore, Tanina& Deubel, Stefanie& Grune, Tilman& Ott, Christiane. Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle Type. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1204643
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204643