H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins
Joint Authors
Belzile, Nelson
Yang, Guangdong
Pei, Yanxi
Wu, Bo
Greasley, Adam
Zhang, Yanjie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Cardiac hypertrophy is defined as the enlargement of the cardiac myocytes, leading to improper nourishment and oxygen supply due to the increased functional demand.
This increased stress on the cardiac system commonly leads to myocardial infarction, contributing to 85% of all cardiac-related deaths.
Cystathionine gamma-lyase- (CSE-) derived H2S is a novel gasotransmitter and plays a critical role in the preservation of cardiac functions.
Selenocysteine lyase (SCLY) has been identified to produce H2Se, the selenium homologue of H2S.
Deficiency of selenium is often found in Keshan disease, a congestive cardiomyopathy.
The interaction of H2S and H2Se in cardiac cell hypertrophy has not been explored.
In this study, cell viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Oxidative stress and cell size were observed through immunostaining.
The expression of genes was determined by real-time PCR and western blot.
Here, we demonstrated that incubation of rat cardiac cells (H9C2) with H2O2 lead to increased oxidative stress and cell surface area, which were significantly attenuated by pretreatment of either H2S or H2Se.
H2S incubation induced SCLY/H2Se signaling, which next caused higher expressions and activities of selenoproteins, including glutathione peroxidase and thioredoxin reductase.
Furthermore, deficiency of CSE inhibited the expressions of SCLY and selenoprotein P in mouse heart tissues.
We also found that both H2S and H2Se stimulated Nrf2-targeted downstream genes.
These data suggests that H2S protects against cardiac hypertrophy through enhancement of a group of antioxidant proteins.
American Psychological Association (APA)
Greasley, Adam& Zhang, Yanjie& Wu, Bo& Pei, Yanxi& Belzile, Nelson& Yang, Guangdong. 2019. H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1204661
Modern Language Association (MLA)
Greasley, Adam…[et al.]. H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1204661
American Medical Association (AMA)
Greasley, Adam& Zhang, Yanjie& Wu, Bo& Pei, Yanxi& Belzile, Nelson& Yang, Guangdong. H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1204661
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204661