Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway
Joint Authors
Li, Cong
Sun, Yang
Zhao, Kun
Leng, Bin
Lu, Mei-Li
Wang, Hong-Xin
Zhang, Ling
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-15
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Vascular endothelial dysfunction is associated with increased mortality in patients with diabetes.
Astragaloside IV (As-IV) is a bioactive saponin with therapeutic potential as an anti-inflammatory and antiendothelial dysfunction.
However, the underlying mechanism for how As-IV ameliorated endothelial dysfunction is still unclear.
Therefore, in this study, we examined the protective effect of As-IV against endothelial dysfunction and explored potential molecular biology mechanism.
In vivo, rats were intraperitoneally injected with streptozotocin (STZ) at a dose of 65 mg/kg body weight to establish a diabetic model.
In vitro studies, rat aortic endothelial cells (RAOEC) were pretreated with As-IV, SB203580 (p38 MAPK inhibitor) for 2 h prior to the addition of high glucose (33 mM glucose).
Our findings indicated that As-IV improved impaired endothelium-dependent relaxation and increased the levels of endothelial NO synthase (eNOS) and nitric oxide (NO) both in vivo and in vitro.
Besides, As-IV treatment inhibited the elevated inflammation and oxidative stress in diabetic model both in vivo and in vitro.
Moreover, As-IV administration reversed the upregulated expression of P2X7R and p-p38 MAPK in vivo and in vitro.
Additionally, the effects of both P2X7R siRNA and SB203580 on endothelial cells were similar to As-IV.
Collectively, our study demonstrated that As-IV rescued endothelial dysfunction induced by high glucose via inhibition of P2X7R dependent p38 MAPK signaling pathway.
This provides a theoretical basis for the further study of the vascular endothelial protective effects of As-IV.
American Psychological Association (APA)
Leng, Bin& Li, Cong& Sun, Yang& Zhao, Kun& Zhang, Ling& Lu, Mei-Li…[et al.]. 2020. Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1204717
Modern Language Association (MLA)
Leng, Bin…[et al.]. Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1204717
American Medical Association (AMA)
Leng, Bin& Li, Cong& Sun, Yang& Zhao, Kun& Zhang, Ling& Lu, Mei-Li…[et al.]. Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1204717
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204717