miR-146a-5p Mediates Intermittent Hypoxia-Induced Injury in H9c2 Cells by Targeting XIAP
Joint Authors
Chen, Qingshi
Lin, Guofu
Chen, Lida
Huang, Jiefeng
Huang, Yaping
Lin, Qichang
Feng, Dehuai
Zhang, Shuyi
Huang, Xiaoyun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
MicroRNAs (miRNAs) have emerged as key modulators in the pathophysiologic processes of cardiovascular diseases.
However, its function in cardiac injury induced by obstructive sleep apnea (OSA) remains unknown.
The aim of the current study was to identify the effect and potential molecular mechanism of miR-146a-5p in intermittent hypoxia(IH)- induced myocardial damage.
We exposed H9c2 cells to IH condition; the expression levels of miR-146a-5p were detected by RT-qPCR.
Cell viability, cell apoptosis, and the expressions of apoptosis-associated proteins were assessed via Cell Counting Kit-8 (CCK-8), flow cytometry, and western blotting, respectively.
Target genes of miR-146a-5p were confirmed by dual-luciferase reporter assay.
IH remarkably lowered viability but enhanced cell apoptosis.
Concomitantly, the miR-146a-5p expression level was increased in H9c2 cells after IH.
Subsequent experiments showed that IH-induced injury was alleviated through miR-146a-5p silence.
X-linked inhibitor of apoptosis protein (XIAP) was predicted by bioinformatics analysis and further confirmed as a direct target gene of miR-146a-5p.
Surprisingly, the effect of miR-146a-5p inhibition under IH may be reversed by downregulating XIAP expression.
In conclusion, our results demonstrated that miR-146a-5p could attenuate viability and promote the apoptosis of H9c2 by targeting XIAP, thus aggravating the H9c2 cell injury induced by IH, which could enhance our understanding of the mechanisms for OSA-associated cardiac injury.
American Psychological Association (APA)
Lin, Guofu& Huang, Jiefeng& Chen, Qingshi& Chen, Lida& Feng, Dehuai& Zhang, Shuyi…[et al.]. 2019. miR-146a-5p Mediates Intermittent Hypoxia-Induced Injury in H9c2 Cells by Targeting XIAP. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204722
Modern Language Association (MLA)
Lin, Guofu…[et al.]. miR-146a-5p Mediates Intermittent Hypoxia-Induced Injury in H9c2 Cells by Targeting XIAP. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1204722
American Medical Association (AMA)
Lin, Guofu& Huang, Jiefeng& Chen, Qingshi& Chen, Lida& Feng, Dehuai& Zhang, Shuyi…[et al.]. miR-146a-5p Mediates Intermittent Hypoxia-Induced Injury in H9c2 Cells by Targeting XIAP. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204722