Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species
Joint Authors
Liu, Cui Hua
Huang, Zhe Hao
Dong, Xin Yu
Zhang, Xin Qiang
Li, Yuan Hang
Zhao, Gang
Sun, Bao Sheng
Shen, Yan Nan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objective.
The mechanism of enhanced radiosensitivity induced by mitochondrial uncoupling protein UCP2 was investigated in HeLa cells to provide a theoretical basis as a novel target for cervical cancer treatment.
Methods.
HeLa cells were irradiated with 4 Gy X-radiation at 1.0 Gy/min.
The expression of UCP2 mRNA and protein was assayed by real-time quantitative polymerase chain reaction and western blotting.
UCP2 siRNA and negative control siRNA fragments were constructed and transfected into HeLa cells 24 h after irradiation.
The effect of UCP2 silencing and irradiation on HeLa cells was determined by colony formation, CCK-8 cell viability, γH2AX immunofluorescence assay of DNA damage, Annexin V-FITC/PI apoptosis assay, and propidium iodide cell cycle assay.
The effects on mitochondrial structure and function were investigated with fluorescent probes including dichlorodihydrofluorescein diacetate (DCFH-DA) assay of reactive oxygen species (ROS), rhodamine 123, and MitoTracker Green assay of mitochondrial structure and function.
Results.
Irradiation upregulated UCP2 expression, and UCP2 knockdown decreased the survival of irradiated HeLa cells.
UCP2 silencing sensitized HeLa cells to irradiation-induced DNA damage and led to increased apoptosis, cell cycle arrest in G2/M, and increased mitochondrial ROS.
Increased radiosensitivity was associated with an activation of P53, decreased Bcl-2, Bcl-xl, cyclin B, CDC2, Ku70, and Rad51 expression, and increased Apaf-1, cytochrome c, caspase-3, and caspase-9 expression.
Conclusions.
UCP2 inhibition augmented the radiosensitivity of cervical cancer cells, and it may be a potential target of radiotherapy of advanced cervical cancer.
American Psychological Association (APA)
Liu, Cui Hua& Huang, Zhe Hao& Dong, Xin Yu& Zhang, Xin Qiang& Li, Yuan Hang& Zhao, Gang…[et al.]. 2020. Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204729
Modern Language Association (MLA)
Liu, Cui Hua…[et al.]. Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1204729
American Medical Association (AMA)
Liu, Cui Hua& Huang, Zhe Hao& Dong, Xin Yu& Zhang, Xin Qiang& Li, Yuan Hang& Zhao, Gang…[et al.]. Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204729
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204729