Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
Joint Authors
Wu, Dongdong
Li, Yanzhang
Li, Jianmei
Zhang, Qianqian
Tian, Wenke
Zhong, Peiyu
Liu, Zhengguo
Wang, Huijuan
Wang, Honggang
Ji, Ailing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-16
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Hydrogen sulfide (H2S) is involved in the development and progression of many types of cancer.
However, the effect and mechanism of H2S on the growth of human thyroid carcinoma cells remain unknown.
In the present study, we found that the proliferation, viability, migration, and invasion of human thyroid carcinoma cells were enhanced by 25–50 μM NaHS (an H2S donor) and inhibited by 200 μM NaHS.
However, H2S showed no obvious effects on the proliferation, viability, and migration of human normal thyroid cells.
Administration of 50 μM NaHS increased the expression levels of CBS, SQR, and TST, while 200 μM NaHS showed reverse effects in human thyroid carcinoma cells.
After treatment with 25-50 μM NaHS, the ROS levels were decreased and the protein levels of p-PI3K, p-AKT, p-mTOR, H-RAS, p-RAF, p-MEK1/2, and p-ERK1/2 were increased, whereas 200 μM NaHS exerted opposite effects in human thyroid carcinoma cells.
Furthermore, 1.4-2.8 mg/kg/day NaHS promoted the tumor growth and blood vessel formation in human thyroid carcinoma xenograft tumors, while 11.2 mg/kg/day NaHS inhibited the tumor growth and angiogenesis.
In conclusion, our results demonstrate that exogenous H2S regulates the growth of human thyroid carcinoma cells through ROS/PI3K/Akt/mTOR and RAS/RAF/MEK/ERK signaling pathways.
Novel H2S-releasing donors/drugs can be designed and applied for the treatment of thyroid cancer.
American Psychological Association (APA)
Wu, Dongdong& Li, Jianmei& Zhang, Qianqian& Tian, Wenke& Zhong, Peiyu& Liu, Zhengguo…[et al.]. 2019. Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1204911
Modern Language Association (MLA)
Wu, Dongdong…[et al.]. Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1204911
American Medical Association (AMA)
Wu, Dongdong& Li, Jianmei& Zhang, Qianqian& Tian, Wenke& Zhong, Peiyu& Liu, Zhengguo…[et al.]. Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1204911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204911