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Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes
Joint Authors
Wende, Kristian
von Woedtke, Thomas
Bekeschus, Sander
Schmidt, Anke
Hasse, Sybille
Jarick, Katja
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-13
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Small reactive oxygen and nitrogen species (ROS/RNS) driven signaling plays a significant role in wound healing processes by controlling cell functionality and wound phase transitions.
The application of cold atmospheric pressure plasma (CAP), a partially ionized gas expelling a variety of ROS and RNS, was shown to be effective in chronic wound management and contrastingly also in malignant diseases.
The underlying molecular mechanisms are not well understood but redox signaling events are involved.
As a central player, the cellular tumor antigen p53 governs regulatory networks controlling proliferation, death, or metabolism, all of which are grossly modulated by anti- and prooxidant signals.
Using a human skin cell model, a transient phosphorylation and nuclear translocation of p53, preceded by the phosphorylation of upstream serine- (ATM) and serine/threonine-protein kinase (ATR), was detected after CAP treatment.
Results indicate that ATM acts as a direct redox sensor without relevant contribution of phosphorylation of the histone A2X, a marker of DNA damage.
Downstream events are the activation of checkpoint kinases Chk1/2 and several mitogen-activated (MAP) kinases.
Subsequently, the expression of MAP kinase signaling effectors (e.g., heat shock protein Hsp27), epithelium derived growth factors, and cytokines (Interleukins 6 + 8) was increased.
A number of p53 downstream effectors pointed at a decrease of cell growth due to DNA repair processes.
In summary, CAP treatment led to an activation of cell repair and defense mechanisms including a modulation of paracrine inflammatory signals emphasizing the role of prooxidant species in CAP-related cell signaling.
American Psychological Association (APA)
Schmidt, Anke& Bekeschus, Sander& Jarick, Katja& Hasse, Sybille& von Woedtke, Thomas& Wende, Kristian. 2019. Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1204928
Modern Language Association (MLA)
Schmidt, Anke…[et al.]. Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1204928
American Medical Association (AMA)
Schmidt, Anke& Bekeschus, Sander& Jarick, Katja& Hasse, Sybille& von Woedtke, Thomas& Wende, Kristian. Cold Physical Plasma Modulates p53 and Mitogen-Activated Protein Kinase Signaling in Keratinocytes. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1204928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204928