Hypoxia Helps Maintain Nucleus Pulposus Homeostasis by Balancing Autophagy and Apoptosis
Joint Authors
Kim, Han-Jun
Lee, Hye-Rim
Kim, Hyosung
Do, Sun Hee
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Intervertebral disc degeneration (IVDD) is a common cause of lower back pain.
Programmed cell death (PCD) including apoptosis and autophagy is known to play key mechanistic roles in the development of IVDD.
We hypothesized that the nucleus pulposus cells that make up the center of the IVD can be affected by aging and environmental oxygen concentration, thus affecting the development of IVDD.
Here, we evaluated the phenotype changes and PCD signaling in nucleus pulposus cells in two different oxygen percentages (5% (hypoxia) and 20% (normoxia)) up to serial passage 20.
NP cells were isolated from the lumbar discs of rats, and the chondrogenic, autophagic, and apoptotic gene expressions were analyzed during cell culture up to serial passage 20.
Hypoxia significantly increased the number of autophagosomes, as determined by monodansylcadaverine staining and transmission electron microscopy.
Furthermore, hypoxia triggered the activation of autophagic flux (beclin-1, LC3-II/LC3-I ratio, and SIRT1) with a concomitant decrease in the expression of apoptotic proteins (Bax and caspase-3).
Despite injury and age differences, no significant differences were observed between the ex vivo lumbar disc cultures of groups incubated in the hypoxic chamber.
Our study provides a better understanding of autophagy- and apoptosis-related senescence in NP cells.
These results also provide insight into the effects of aging on NP cells and their PCD levels during aging.
American Psychological Association (APA)
Kim, Han-Jun& Lee, Hye-Rim& Kim, Hyosung& Do, Sun Hee. 2020. Hypoxia Helps Maintain Nucleus Pulposus Homeostasis by Balancing Autophagy and Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204981
Modern Language Association (MLA)
Kim, Han-Jun…[et al.]. Hypoxia Helps Maintain Nucleus Pulposus Homeostasis by Balancing Autophagy and Apoptosis. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1204981
American Medical Association (AMA)
Kim, Han-Jun& Lee, Hye-Rim& Kim, Hyosung& Do, Sun Hee. Hypoxia Helps Maintain Nucleus Pulposus Homeostasis by Balancing Autophagy and Apoptosis. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1204981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204981