CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice
Joint Authors
Zuo, Zhi
Li, Peng
Ji, Ming-Yue
Zhao, Kun
Su, Zhong-Ping
Hou, Dao-Rong
Li, Yong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-19
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In this study, we investigated whether CD47 deficiency attenuates isoproterenol- (ISO-) induced cardiac remodeling in mice.
Cardiac remodeling was induced by intraperitoneal (i.p.) injection of ISO (60 mg·kg-1·d-1 in 100 μl of sterile normal saline) daily for 14 days and was confirmed by increased levels of lactate dehydrogenase (LDH) and creatine kinase MB (CK-MB), increased heart weight to body weight (HW/BW) ratios, and visible cardiac fibrosis.
Apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining.
Levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were found to be significantly higher in the ISO group than in the control group, while superoxide dismutase (SOD) levels were suppressed in the ISO group.
However, CD47 knockout significantly limited ISO-induced increases in LDH, CK-MB, and HW/BW ratios, cardiac fibrosis, oxidative stress, and apoptosis in the heart.
In addition, CD47 deficiency also increased p-AMPK and LAMP2 expression and decreased HDAC3, cleaved Caspase-3, cleaved Caspase-9, LC3II, and p62 expression in cardiac tissues.
In conclusion, CD47 deficiency reduced i.p.
ISO-induced cardiac remodeling probably by inhibiting the HDAC3 pathway, improving AMPK signaling and autophagy flux, and rescuing autophagic clearance.
American Psychological Association (APA)
Zuo, Zhi& Ji, Ming-Yue& Zhao, Kun& Su, Zhong-Ping& Li, Peng& Hou, Dao-Rong…[et al.]. 2019. CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1205005
Modern Language Association (MLA)
Zuo, Zhi…[et al.]. CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1205005
American Medical Association (AMA)
Zuo, Zhi& Ji, Ming-Yue& Zhao, Kun& Su, Zhong-Ping& Li, Peng& Hou, Dao-Rong…[et al.]. CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1205005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205005