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Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network
Joint Authors
Cheng, Xin
Yi, Min
Zhang, Zheyu
Yi, Pengji
Hu, Yang
Tong, Xiaoliang
Peng, Weijun
Yang, Jingjing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-02
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
Quercetin is a bioflavonoid with potential antioxidant properties.
However, the mechanisms underlying its effects remain unclear.
Herein, we focused on integrating long noncoding RNA (lncRNA), microRNA (miRNA), and messenger RNA (mRNA) sequencing of PC-12 cells treated with quercetin.
We treated PC-12 cells with hydrogen peroxide to generate a validated oxidative damage model.
We evaluated the effects of quercetin on PC-12 cells and established the lncRNA, miRNA, and mRNA profiles of these cells.
Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of these RNAs were conducted to identify the key pathways.
Quercetin significantly protected PC-12 neuronal cells from hydrogen peroxide-induced death.
We identified 297, 194, and 14 significantly dysregulated lncRNAs, miRNAs, and mRNAs, respectively, associated with the antioxidant effect of quercetin.
Furthermore, the phosphatidylinositol-3-kinase/protein kinase B pathway was identified as the crucial signalling pathway.
Finally, we constructed a lncRNA-associated competing endogenous RNA (ceRNA) network by utilizing oxidative damage mechanism-matched miRNA, lncRNA, and mRNA expression profiles and those changed by quercetin.
In conclusion, quercetin exerted a protective effect against oxidative stress-induced damage in PC-12 cells.
Our study provides novel insight into ceRNA-mediated gene regulation in the progression of oxidative damage and the action mechanisms of quercetin.
American Psychological Association (APA)
Zhang, Zheyu& Yi, Pengji& Yi, Min& Tong, Xiaoliang& Cheng, Xin& Yang, Jingjing…[et al.]. 2020. Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1205012
Modern Language Association (MLA)
Zhang, Zheyu…[et al.]. Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-22.
https://search.emarefa.net/detail/BIM-1205012
American Medical Association (AMA)
Zhang, Zheyu& Yi, Pengji& Yi, Min& Tong, Xiaoliang& Cheng, Xin& Yang, Jingjing…[et al.]. Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1205012
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205012